Effect of Mitomycin C on the Proliferation and Nitric Oxide Production in the Cultured Trabecular Meshwork Cells.

Cho, Beum Joon; Kim, Jae Woo

Original Article

Journal of the Korean Ophthalmological Society 2003;44(2):485-490.
Published online February 1, 2003.

Effect of Mitomycin C on the Proliferation and Nitric Oxide Production in the Cultured Trabecular Meshwork Cells.

Beum Joon Cho, Jae Woo Kim

Department of Ophthalmology, School of Medicine, Catholic University of Daegu, Korea. jwkim@cataegu.ac.kr

마이토마이신 C가 배양된 섬유주세포의 증식과 NO의 생성에 미치는 영향

조범준 ( Beum Joon Cho ) , 김재우 ( Jae Woo Kim )

Abstract

PURPOSE
To investigate the effect of mitomycin C on the proliferation of cultured trabecular cells and its relation to nitric oxide (NO) production. METHODS: The effect of NO donor, SIN-1, on the proliferation of primarily cultured porcine trabecular meshwork cells was studied with MTT assay. After treatment with mitomycin C at various concentrations for 1 hour, the proliferation was assessed by MTT assay and the production of nitrite was assessed by Griess reaction after 24 hours and 3 days respectively. RESULTS: SIN-1 significantly inhibited proliferation of cultured trabecular meshwork cells (p<0.05). Mitomycin C also suppressed the cellular proliferation(p<0.05) but did not affect the production of nitrite (p>0.05). CONCLUSIONS: Both NO donor and mitomycin C have an inhibitory effect on the proliferation of trabecular meshwork cells. However, inhibitory effect of mitomycin C was not related to the production of NO.

Key Words: Culture;Griess reaction;Mitomycin C;MTT;Nitric oxide;Proliferation;Trabecular meshwork cells