J Korean Ophthalmol Soc > Volume 58(10); 2017 > Article
Journal of the Korean Ophthalmological Society 2017;58(10):1155-1159.
DOI: https://doi.org/10.3341/jkos.2017.58.10.1155    Published online October 15, 2017.
The Effect of Anti-inflammatory Agents on the Permeability of Trabecular Meshwork Cell Monolayers.
Hye Ri Kang, Jung Hoon Lee, Jae Woo Kim
1Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr
2Catholic Union Eye Clinic, Daegu, Korea.
소염제가 섬유주단층세포층의 투과성에 미치는 효과
강혜리1⋅이정훈2⋅김재우1
대구가톨릭대학교 의과대학 안과학교실1, 가톨릭연합안과의원2
Correspondence:  Jae Woo Kim,
Email: jwkim@cu.ac.kr
Received: 13 October 2016   • Revised: 5 January 2017   • Accepted: 18 September 2017
Abstract
PURPOSE
To compare the effects of anti-inflammatory agents, specifically bromfenac, loteprednol, and prednisolone, on the permeability of cultured human trabecular meshwork cell (HTMC) monolayers. METHODS: HTMCs were cultured until confluency in the inner chamber of Transwell, then exposed to 1/1,000 or 1/500 diluted commercial 0.1% bromfenac, 0.5% loteprednol, and 1% prednisolone for 24 hours. The permeabilities of carboxyfluorescein through the HTMC monolayer were measured with a spectrofluorometer after 2 hours in the outer chamber. Cellular viabilities were assessed with an 3-[4,5–dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. RESULTS: Bromfenac and loteprednol diluted at 1/1,000 or 1/500 did not significantly affect the cellular survival (p > 0.05). Bromfenac did not affect the permeability via the HTMC monolayer (p > 0.05) and loteprednol decreased the permeability (p < 0.05). In addition, 1/2,000 prednisolone also decreased the permeability (p < 0.05). CONCLUSIONS: Among the anti-inflammatory agents, the non-steroidal anti-inflammatory agent bromfenac did not affect the permeability, while loteprednol and prednisolone decreased the permeability through the HTMC monolayer. Thus, loteprednol and prednisolone may decrease the trabecular outflow.
Key Words: Bromfenac;Loteprednol;Permeability;Prednisolone;Trabecular meshwork cells


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