| Effect of Tetrahydrozoline on the Permeability of Trabecular Meshwork Cell Monolayer. |
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Seok Jin Hwang, Jae Woo Kim |
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Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr |
| 테트라히드로졸린이 섬유주단층세포층의 투과도에 미치는 영향 |
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황석진⋅김재우 |
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대구가톨릭대학교 의과대학 안과학교실 |
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| Abstract |
PURPOSE
To investigate the effects of tetrahydrozoline (THZ) on the survival of cultured human trabecular meshwork cells (HTMC) and the permeability of HTMC monolayer. METHODS: Primary cultured HTMC were exposed to an adrenergic agonist (0.01, 0.1, 1.0 or 10 µM THZ) for 1 day and 3 days. Carboxyfluorescein permeability through the HTMC monolayer was measured using Transwell. Cellular viability and nitric oxide (NO) production were assessed using MTT and Griess assays, respectively. RESULTS: THZ did not affect the cellular survival (p > 0.05) or NO production (p > 0.05). THZ significantly increased the carboxyfluorescein permeability through the HTMC monolayer in a dose-dependent manner compared with non-exposed control (p < 0.05) after exposure for 1 and 3 days. CONCLUSIONS: THZ does not affect the survival of HTMC but decreases the permeability of HTMC monolayer in a dose-dependent manner. Thus, THZ may possibly decrease trabecular outflow. |
| Key Words:
Permeability;Survival;Tetrahydrozoline;Trabecular meshwork cells |
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