J Korean Ophthalmol Soc > Volume 52(8); 2011 > Article
Journal of the Korean Ophthalmological Society 2011;52(8):970-974.
DOI: https://doi.org/10.3341/jkos.2011.52.8.970    Published online August 15, 2011.
Effect of Genistein on the Survival and Production of Nitric Oxide in Trabecular Meshwork Cells.
Jung Heum Hong, Yun Young Kim, Jae Woo Kim
Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr
Genistein이 섬유주세포의 생존과 일산화질소의 생성에 미치는 영향
홍정흠⋅김윤영⋅김재우
Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea
Abstract
PURPOSE
To investigate the effects of genistein on the survival and production of nitric oxide in cultured human trabecular meshwork cells (HTMC). METHODS: Primarily cultured HTMC were exposed to 0, 1, and 10 microm genistein using serum-deprived media. Production of nitric oxide and eNOS activity were assessed with the Griess assay and RT-PCR after exposure to genistein for 10 min and one day. Cellular survival was assessed via MTT assay after exposure to genistein for one day. RESULTS: Genistein significantly increased the production of nitric oxide after exposure for 1 min at 10 microm and for 1 day at 1 microm under serum-deprived conditions. Genistein increased eNOS activity and cellular survival in HTMC. CONCLUSIONS: Genistein increases cellular survival under serum-deprived conditions, accompanied with an increase in nitric oxide production after both short-term and long-term exposures.
Key Words: Genistein;Nitric oxide;Survival;Trabecular meshwork cells


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