Role of Nitric Oxide in the Proliferative and Migratory Effect of Triamcinolone in RPE Cells.

Kim, Jae Woo; Lee, Jae Hyung; Lee, Seung Hee

doi:2010.51.1.120

J Korean Ophthalmol Soc > Volume 51(1); 2010 > Article

Original Article

Journal of the Korean Ophthalmological Society 2010;51(1):120-125.
DOI: https://doi.org/10.3341/jkos.2010.51.1.120 Published online January 15, 2010.

Role of Nitric Oxide in the Proliferative and Migratory Effect of Triamcinolone in RPE Cells.

Jae Woo Kim, Jae Hyung Lee, Seung Hee Lee

Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea. jwkim@cu.ac.kr

트리암시놀론의 망막색소상피세포 증식과 이동성 효과에 대한 일산화질소의 역할

김재우ㆍ이재형ㆍ이승희

Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea

Abstract

PURPOSE
To investigate the role of nitric oxide (NO) on the proliferative and migratory effects of triamcinolone acetonide (TA) in retinal pigment epithelial cells. METHODS: After exposure to 10 nM, 1 micrometer, or 100 micrometer TA for four days, with or without co-exposure of antioxidant N-acetylcyteine, the proliferation and nitrite production of ARPE19 cells were assessed with MTT and Griess assays, respectively. Additionally, a cell migration assay was performed. RESULTS: Cellular survival increased after exposure to TA at low concentration but decreased at high concentration. TA decreased the production of NO and cellular migration significantly, and these effects were abolished by N-acetylcysteine. CONCLUSIONS: TA showed a biphasic response on the proliferation and decreased cellular migration in ARPE19 cells, which may be mediated by nitric oxide.

Key Words: ARPE19;Migration;Nitric oxide;Triamcinolone acetonide