Role of Nitric Oxide on the Proliferation of Human Tenon Capsule Fibroblasts.

Kim, Keun Hae; Kim, Jae Woo

Original Article

Journal of the Korean Ophthalmological Society 2003;44(7):1670-1674.
Published online July 1, 2003.

Role of Nitric Oxide on the Proliferation of Human Tenon Capsule Fibroblasts.

Keun Hae Kim, Jae Woo Kim

Department of Ophthalmology, Catholic University of Daegu College of Medicine, Korea. jwkim@cu.ac.kr

테논낭 섬유아세포의 증식에 대한 NO의 역할

김근해 ( Keun Hae Kim ) , 김재우 ( Jae Woo Kim )

Abstract

PURPOSE
To investigate the role of nitric oxide(NO) on the survival and proliferation of human Tenon capsule fibroblasts(HTCF) in tissue culture. METHODS: Following exposure to NO donor such as sodium nitroprusside(SNP) or to NO synthase inhibitor such as N omega-Nitro-L-arginine methyl ester(L-NAME) at various concentrations for 24 hr in the media with or without serum, the cellular proliferation and nitrite production were assessed by rapid colorimetric assay (MTT assay) and Griess assay, respectively. RESULTS: L-NAME decreased the cellular proliferation of HTCF in a dose-dependent manner. In contrast, SNP increased the proliferation in a dose-dependent manner. The differences of nitrite production were more remarkable in the serum-deprived condition with the L-NAME or SNP administration. CONCLUSIONS: NO donor has a proproliferative, and NOS inhibitor has a antiproliferative activities on the proliferation of HTCF. This suggests that NO may be act as an important modulator in wound healing.

Key Words: Fibroblast;Griess;L-NAME;MTT;Nitric oxide;Sodium nitroprusside