Effect of Nitric Oxide on the Survival of R28 Cells.

Lee, Jeong Il; Kim, Jae Woo

doi:2009.50.6.919

J Korean Ophthalmol Soc > Volume 50(6); 2009 > Article

Original Article

Journal of the Korean Ophthalmological Society 2009;50(6):919-922.
DOI: https://doi.org/10.3341/jkos.2009.50.6.919 Published online June 15, 2009.

Effect of Nitric Oxide on the Survival of R28 Cells.

Jeong Il Lee, Jae Woo Kim

Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea. jwkim@cu.ac.kr

일산화질소가 R28 세포의 생존에 미치는 영향

이정일ㆍ김재우

Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea

Abstract

PURPOSE
To evaluate the effect of nitric oxide (NO) on the survival of R28 cells. METHODS: After immunostaining for GFAP, vimentin, S-100, and neurofilament, R28 cells were exposed to S-nitroso-N-acetyl-D, L-penicillamine (SNAP) at various concentrations, with and without the NO inhibitor, Nomega-Nitro-L-arginine methyl ester (L-NAME) for 1 and 3 days. Cellular survival of R28 cells and the production of NO were quantified by rapid colorimetric assays using the MTT and Griess assay, respectively. To evaluate the effect of serum, 10% serum or serum-free media were used separately. RESULTS: R28 cells showed strong immunoreactivity to GFAP and vimentin compared to S-100 or neurofilament. SNAP inhibited the survival of R28 cells in a dose-dependent manner, and this effect of NO on the cellular survival was abolished by L-NAME. These results were similar after exposure for 1 and 3 days, regardless of the presence of serum in the media. CONCLUSIONS: The current results suggest that NO decreased the survival of R28 cells. Further studies are necessary to evaluate the mechanism of cytotoxicity of the R28 cells.

Key Words: Neurotoxicity;Nitric oxide;R28 cells