| Immune Tolerance Induction by Anti-CD4 Monoclonal Antibody in Experimental Autoimmune Anterior Uveitis. |
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Hyeong Gon Yu, Dong Sup Lee, Hum Chung |
1Department of Ophthalmology, Seoul National University, College of Medicine, Korea. chungh@snu.ac.kr
2Department of Anatomy, Seoul National University, College of Medicine, Korea.
3Seoul National University Hospital Clinical Research Institute, Korea. |
| 실험적 자가면역 앞포도막염에서 항CD4항체에 의한 면역관용유도 |
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유형곤 ( Yu Hyeong Gon ) , 이동섭 ( Lee Dong Seob ) , 정흠 ( Jeong Heum ) |
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| Abstract |
PURPOSE
To investigate the therapeutic potential of anti-CD4 antibody as an anti-inflammatory agent for the experimental autoimmune anterior uveitis. METHODS: Lewis rats were immunized with melanin-associated antigen and treated with anti-CD4 antibody for a week and then received secondary immunization with the same antigen at 8 weeks after primary immunization. The clinical grading of the uveitis and the effect of anti-CD4 on cell death and cytokine (IFN-gamma, IL-10) expression were followed. RESULTS: The cell death of CD4+ T cells was dependent on the dose of the antibody. The prevention of the primary uveitis paralleled the deletion of CD4+ T cells. The secondary uveitis was suppressed in the eyes treated early with lower doses (0.1, 1 mg/kg) of the antibody, even though mild degree of intraocular inflammation appeared in the primary uveitis in these groups. In the eyes treated with higher doses (10, 20 mg/kg), the severity of secondary uveitis was slightly reduced despite complete suppression of primary uveitis. IFN-gamma producing CD4+ T cells decreased and IL-10 producing CD4+ T cells increased after early anti-CD4 mAb treatment with a lower dose (1.0 mg/kg). CONCLUSIONS: These results show that anti-CD4 antibody treatment could be used in the future for the treatment of endogenous uveitis. |
| Key Words:
Anti-CD4 antibody;CD4+ T cell;Experimental autoimmune anterior uveitis |
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