| Transcriptional Analysis of Nod-Like Receptors in a Mouse Model of Experimental Autoimmune Uveitis. |
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Hyeong Min Kim, Tae Wan Kim, Hyun Jeong Jeong, Jin Suk Ryu, Mee Kum Kim, Won Ryang Wee, Joo Youn Oh |
1Department of Ophthalmology, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Korea. jooyounoh77@gmail.com
2Department of Ophthalmology, Seoul Metropolitan Government Seoul National University Boramae Medical Center, Seoul, Korea.
3Laboratory of Ocular Regenerative Medicine and Immunology, Seoul Artificial Eye Center, Seoul National University Hospital Biomedical Research Institute, Seoul, Korea. |
| 실험적 자가면역 포도막염 마우스 모델에서 Nod양 수용체 전사체의 안내 발현 양상 분석 |
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김형민1⋅김태완2⋅정현정3⋅류진숙3⋅김미금1,3⋅위원량1,3⋅오주연1,3 |
Department of Ophthalmology, Seoul National University Hospital, Seoul National University College of Medicine1, Seoul, Korea
Department of Ophthalmology, Seoul Metropolitan Government Seoul National University Boramae Medical Center2, Seoul, Korea
Laboratory of Ocular Regenerative Medicine and Immunology, Seoul Artificial Eye Center, Seoul National University Hospital Biomedical
Research Institute3, Seoul, Korea |
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| Abstract |
PURPOSE
To evaluate the transcription pattern of Nod-like receptors (NLRs), the intracellular sensors, to detect danger signals in murine eyes with experimental autoimmune uveitis (EAU). METHODS: EAU was induced in B6 (C57BL/6) mice by subcutaneous injection of human interphotoreceptor retinoid binding protein and intraperitoneal injection of pertussis toxin. At 1, 2, and 3 weeks post-immunization, the eyeballs were extracted and subjected to histological and molecular assays using real-time reverse transcription polymerase chain reaction. RESULTS: The levels of nucleotide-binding oligomerization domain, Leucine rich Repeat and Pyrin domain 1 (NLRP1), NLRP3, nucleotide-binding oligomerization domain-containing protein 1 (NOD1), and NOD2 transcripts were increased at 2 weeks and gradually reduced thereafter. Notably, NLRP3 showed the highest expression in the eyes with EAU. Similarly, the transcript level of pro-inflammatory cytokine, interleukin-1beta, increased and reached a peak at 2 weeks post-immunization. The retinal structure was severely damaged by inflammation at 3 weeks post-immunization. CONCLUSIONS: Among NLRs, NLRP3 may induce inflammation in eyes after EAU immunization. |
| Key Words:
Experimental autoimmune uveitis;NLRP3;Nod-like receptors |
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