| The Effect of Antimetabolites for Inhibiting the Proliferation of Rabbit Lens Epithelial Cells in Vitro. |
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Seung Jeong Lim, Dae Hwi Ahn, yong Sung You, Hong Bok Kim |
1The Institute of Vision Research, College of Medicine, Yonsei University.
2Department of Ophthalmology, Yonsei University,College of Medicine. |
| 항대사성 약제들에 의한 가토 배양수정체 상피세포의 증식억제 효과 |
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임승정(Seung Jeong Lim),안대휘(Dae Hwi Ahn),유용성(Yong Sung You),김홍복(Hong Bok Kim) |
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| Abstract |
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The most common cause of blurred vision after extracapsular cataract extraction is known to be an opacification of the posterior lens capsule. The pathogenesis of posterior lens capsule opacification is primarily caused by residual lens epithelial cells. For the prevention of posterior capsular opacification, several kinds of anti-mitotic drugs is being actively investigated. But the antimitotic drugs are not clinically used due to toxicity towards the intraocular tissues. The objectives of this study is to evaluate the effect of mitomycin C and tirilazad mesylate(FREEDOX(TM)) respectively for inhibiting the proliferation of rabbit lens epithelial cells when it is administered in a short period. Lens epithelial cells from white rabbits were harvested andcultured for 4 passages. Mitomycin C was applied for 3 minutes with 0.025mg/ml and 0.05mg/ml in concentration respectively. The proliferation assay was performed by [(3)H]-thymidine uptake test. Significant decrease of lens epithelial cell proliferation appeared in both drugs.When Mitomycin-C was applied with 0.025mg/ml for 3 minutes, cell proliferation was reduced to 31.5% compared with control and in 0.05mg/ml concentration, to 12.5%. When tirilazad mesylate was applied 0.15mg/ml for 3 minutes, cell proliferation was reduced to 46.5% compared with control and in 1.5mg/ml concentration, to 7.5%. If futher investigation would show the effectives and safety of these drugs, these agents could be applied into the lens capsular bad at the time of surgery to prevent the posterior capsular opacification after cataract surgery. |
| Key Words:
Lens epithelial cell;mitomycin C;Tirilazad mesylate;[3H]-thymidine uptake test |
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