| The Regulation of MMP-2 and -14 Expressions by TGF-beta in Lens Epithelial Cells. |
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Min Jung Son, Jong Tak Kim, Choun Ki Joo |
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Laboratory of Ophthalmology and Visual Science, Catholic Research Institutes of Medical Science, Catholic University Medical College, Seoul, Korea. ckjoo@catholic.ac.kr |
| 수정체 상피세포에서 TGF-β에 의한 MMP-2와 MMP-14의 발현과 조절 기전 |
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손민정,김종탁,주천기 |
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Laboratory of Ophthalmology and Visual Science, Catholic Research Institutes of Medical Science, Catholic University Medical College, Seoul, Korea |
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Correspondence:
Jong Tak Kim, Ph.D. |
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| Abstract |
PURPOSE
TGF-beta is a key regulator of epithelial-mesenchymal transition. Among the TGF-beta responses, cell migration is closely associated with the expression of matrix metalloproteinases (MMPs). Therefore, we determined which MMPs are regulated by TGF-beta and examined the TGF-beta signaling involved in this event, focusing on Src family tyrosine kinases (SFKs) METHODS: First we examined the expression of MMPs in rat lens explant culture treated with TGF-beta and LECs attached to the anterior capsules of patients with nuclear (N), anterior polar (AP) cataracts using RT-PCR and immunofluorescence staining. It was examined whether the expression of MMPs is regulated by SFKs. RESULTS: The study using RT-PCR and immunofluorescence staining showed the expression of MMP-2 and -14 in explants and the expression of MMP-14 LECs of AP cataracts. The expression of MMP-2 and -14 was blocked by PP2 in explants. Furthermore, the activated form of SFKs was observed in LECs of AP cataracts by immunofluorescence staining. CONCLUSIONS: We suggest a novel role of SFKs signaling in the expression of MMP-14 induced by TGF-beta. |
| Key Words:
Lens epithelial cell;Matrix metalloproteinase;Nuclear and anterior polar cataract;Transforming growth factor-beta |
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