| The Effects of Glucose Concentrations on Reactive Oxygen products in Human Lens Epithelial Cells. |
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Yoo Ri Yang, Bypung Rai Lee, Jae Woong Koh |
1Department of ophthalmology, Seoul Veterans Hospital, Seoul, Korea.
2Department of Biochemistry, Chosun university College of Medicine, Gwangju, Korea.
3Department of ophthalmology, Chosun university College of Medicine, Gwangju, Korea. clearcornea@paran.com |
| 포도당 농도가 인간 수정체상피세포의 반응성 산소 생성량의 변화에 미치는 영향 |
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양유리1,이병래2,고재웅3 |
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Department of ophthalmology, Seoul Veterans Hospital1, Seoul, Korea Department of Biochemistry, Chosun university College of Medicine2, Gwangju, Korea Department of ophthalmology, Chosun university College of Medicine3, Gwangju, Korea |
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Correspondence:
Yoo Ri Yang, M.D.1 |
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| Abstract |
PURPOSE
To investigate the effects of glucose concentrations on the formation of reactive oxygen products and cellular injury in human lens epithelial (HLE) cells. METHODS: Human lens epithelial (HLE) cells were cultured with high glucose (200 mg/100 ml, 300 mg/100 ml, 400 mg/100 ml) and normal glucose (100 mg/100 ml) contained Modified Egle's Medium (MEM). The amounts of reactive oxygen products were assayed by dihydroethidium (DHE). Paraquat-induced cytotoxicity was determined by the 3-[4,5-dimethylthiazol-2-yl]-2,5 -diphenyl tetrazolium bromide (MTT) method. RESULTS Reactive oxygen products of human lens epithelial cells increased 110%, 250% and 630% in the high glucose (200 mg/100 ml, 300 mg/100 ml, 400 mg/100 ml) media compared to the normal glucose (100 mg/100 ml) media. The paraquat-induced cells toxicity was increased by the high glucose. CONCLUSIONS: High glucose increased the formation of reactive oxygen products in human lens epithelial cells. These results suggest that high glucose can induce cataract formation in diabetic patients. |
| Key Words:
High Glucose;Human Lens Epithelial Cells;Reactive Oxygen |
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