| Effect of Vascular Endothelial Growth Factor(VEGF) on the Corneal Neovascularization and Expression of MMP-2, 9, TIMP-1, 2 and flk-1. |
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Jae Bum Lee, Seung Eun Jung, Hyun Chae Lee, Hyuck Moon Kwon, Bum Kee Hong, Hyun Young Park, Eung Kweon Kim, Jung Hyub Oh |
1Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea.
2The Institute of Vision Research, Yonsei University College of Medicine, Seoul, Korea. jblee88@yumc.yonsei.ac.kr
3Department of Internal medicine, Cardiovascular research institute, Yonsei University College of Medicine, Seoul, Korea.
4Department of Ophthalmology, Inha University College of Medicine, Incheon, Korea. |
| VEGF ( Vascular endothelial growth factor ) 에 의한 각막신생혈관 생성 및 MMP - 2 , 9 , TIMP - 1 , 2 , flk - 1 의 발현 |
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오중협(Jung Hyab Oh),이재범(Jae Bum Lee),정승은(Seung Eun Jung),이현채(Hyun Chae Lee),권혁문(Hyuck Moon Kwon),홍범기(Bum Kee Hong),박현영(Hyun Young Park),김응권(Eung Kweon Kim) |
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| Abstract |
PURPOSE
The purpose of this study is to clarify the degree of corneal neovascularization and its expression of MMP-2. 9(matrix metalloproteinase) and TIMP-1, 2(tissue inhibitor of metalloproteinases) according to the different concentrations of VEGF(vascular endothelial growth factor). METHODS: After the pellets with different amounts of VEGF(VEGF 125, 250 ng) were inserted into the corneal stroma of rat model, their degree of corneal neovascularization and the expression of MMP-2, 9 and TIMP-1, 2 were compared with those of control group where pellets were filled with phosphate-buffered saline. RESULTS: At the 7th day after the pellet insertion, degree of neovascularization was most highly scored in the group with pellet which contained the largest amount of VEGF, 250 ng, and there was statistically noticeable increase of eovascularization with the increase of VEGF amount(P<0.05). On immunohistochemical staining, as the amount of VEGF increases, not only MMP-2, 9 and flk-1, but also TIMP-1, 2 were expressed more. CONCLUSIONS: In conclusion, when it comes to neovascularization, MMP-2, 9, which induces angiogenesis, as well as its inhibitor TIMP-1, 2 are increased to maintain the homeostasis of the cornea. |
| Key Words:
Corneal neovascularization;flk-1;MMP-2, 9;TIMP-1, 2;VEGF |
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