| Change of Surface Carbohyd rate during Trans differentiation of Retinal Pigment Epithelial Cell. |
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Hee Seong Yoon, Sung Chur Moon, Woo Jin Jeung, Hae Il Park, Young Hyun Yoo |
1Department of Ophthalmology, College of Medicine, Dong-A University, Pusan, Korea.
2Department of Anatomy and Institute of Medical Science, College of Medicine, Dong-A University, Pusan, Korea. |
| 망막색소상피의 Transdifferentiation 동안 세포표면 탄수화물의 변화 |
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윤희성(Hee Seong Yoon),문성철(Sung Chur Moon),정우진(Woo Jin Jeung),박해일(Hae Il Park),유영현(Young Hyun Yoo) |
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| Abstract |
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This study was conducted to study the changes of cell surface carbohydrates during transdifferentiation of retinal pigment epithelial(RPE)cells. RPE cells were cultured from adult pig eyes. Surface carbohydrates of RPE cells from 1st, 3rd, 5th, 7th and 9th passages were assayed by lectin histochemistry and enzyme immunoassay. Changes in binding affinities to the lectins employed were demonsrated during trasdifferentiation of RPE cell. Whereas binding affinities of ConA, ECL, PNA, WGA, and UEA-I decreased graudally as the number of culture passage increased, binding properties to LCA, STL and DBA fluctuated depending on the number of passages. The results demonstrate changes of surface carbohydrates of RPE cell during trasdifferentiation. We suggest that changes of surface carbohydrates of RPE cell during trasdifferentiation may be close relations with the functional changes during transdifferentiation. |
| Key Words:
Lectin;RPE cell;Surface carbohydrate;Transdifferentiation |
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