J Korean Ophthalmol Soc > Volume 50(4); 2009 > Article
Journal of the Korean Ophthalmological Society 2009;50(4):594-602.
DOI: https://doi.org/10.3341/jkos.2009.50.4.594    Published online April 30, 2009.
Effect of Triamcinolone on Angiogenesis-related Factors of Cultured Retinal Pigment Epithelial Cells.
Young Chang Lee, Tae Jung Yoon, Gwang Ju Choi, Dae Hyun Kim
1Department of Ophthalmology, College of Medicine, Chosun University, Gwangju, Korea. eyelovehyun@hanmail.net
2I Clinic, Gwangju, Korea.
트리암시놀론이 배양망막상피세포에서 생산된 혈관형성관련인자에 미치는 영향
이영창1ㆍ윤태중2ㆍ최광주1ㆍ김대현1
Department of Ophthalmology, College of Medicine, Chosun University1, Gwangju, Korea I Clinic2, Gwangju, Korea
Abstract
PURPOSE
To examine the effects of triamcinolone on angiogenesis related factors in cultured human retinal pigment epithelial cells. METHODS: Human retinal pigment epithelial cells were exposed to triamcinolone, cultured in a hypoxic environment, and expression and production of VEGF and PEDF were subsequently tested by RT-PCR and Western blot. Angiogenesis was measured via a tube formation assay using ECV 304 cells and with a migration assay using human dermal microvascular endothelial cells. RESULTS: Expression and production of VEGF and PEDF were tested by RT-PCR and Western blot, respectively. VEGF abundance was reduced while that of PEDF was unchanged in triamcinolone exposed retinal pigment epithelial cells cultured in hypoxic environment compared with cells with no treatment in hypoxic environment (p<0.05). Tube formation and cell migration were reduced by triamcinolone (p<0.05). CONCLUSIONS: These results suggest that triamcinolone affects the secretion of angiogenesis-related factors and suppresses neovascularization.
Key Words: Angiogenesis;PDEF;Triamcinolone;VEGF


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