The Effect of Hyaluronic Acid and Epidermal Growth Factor on Cultured Corneal Epithelial Cells in the Rabbit.

Lee, Eun Kyung; Jin, Kyung Hyun; Kim, Jae Myung

Original Article

Journal of the Korean Ophthalmological Society 1993;34(10):978-984.
Published online October 1, 1993.

The Effect of Hyaluronic Acid and Epidermal Growth Factor on Cultured Corneal Epithelial Cells in the Rabbit.

Eun Kyung Lee, Kyung Hyun Jin, Jae Myung Kim

Department of Ophthalmology, Kyung Hee University, College of Medicine, Seoul, Korea.

각막 상피세포 배양시 Hyaluronic Acid 와 Epidermal Growth Factor 의 효과

이은경(Eun Kyung Lee),진경현(Kyung Hyun Jin),김재명(Jae Myung Kim)

Abstract

Corneal epithelial wound healing is followed by cellular migration and proliferation. Hyaluronic acid and EGF have been used to promote corneal wound healing. We investigated the effect and action mechanism of Hyaluronic acid and EGF using agarose explant method and organ culture. In the agarose explant method, we studied the promoting effect of different concentration of Hyaluronic acid and EGF, combined effects and mitotic inhibitory effect of colchicine on cultured corneal epithelial cells in the rabbit. And we studied the result of the organ culture with same agents using round epithelial defect by the trephine. Hyaluronic acid and EGF showed significant promoting effect on corneal wound healing, and the combined effect was also significant. The effect of Hyaluronic acid and EGF was inhibited in colchicine combined culture. Therefore, the effect of hyaluronic acid and EGF is seemed to be on the cell proliferation rather than cell motility.

Key Words: Colchicine;Cornea;Culture;Epidermal Growth Factor;Hyaluronic acid