The Effect of Lens Nucleus and Cortex Material on Lens Epithelial Cell Culture through In vitro Capsular Bag Model.

Lee, Jaehwan; Chin, Heeseung; Oh, Junghyub

Original Article

Journal of the Korean Ophthalmological Society 2001;42(4):630-637.
Published online April 1, 2001.

The Effect of Lens Nucleus and Cortex Material on Lens Epithelial Cell Culture through In vitro Capsular Bag Model.

Jaehwan Lee, Heeseung Chin, Junghyub Oh

Department of Ophthalmology, Medical College, Inha University.

수정체 핵 및 피질 성분이 In vitro Capsular Bag Model 의 수정체상피세포 배양에 미치는 영향

이재환(Jae Hwan Lee),진희승(Hee Seung Chin),오중협(Jung Hyub Oh)

Abstract

PURPOSE
This study attempts to evaluate the effect of lens cortex and nucleus remnants on posterior capsular opacification with method of the cell culture according to in vitro capsular bag model. METHODS: After bovine lens were isolated, we performed continuous curvilinear capsulorhexis and hydrodissectioin of the lens fiber mass. At this stage a tension ring was implanted and then the preparations placed in organ culture for up to 6 weeks. Lens cortex and nucleus material was added at the culture media in group 2, 3, 4, 5, 6 with amount of 1/16, 1/32, 1/64, 1/96, 1/128 of one lens volume. Group 1 was control group that was not added lens materials. Cell coverage of the posterior lens capsule was recorded and the capsules were examined, both pre-and post-coverage, for proliferative activity. RESULTS: After a lag period outgrowth was observed across the posterior capsule. The proliferative activity was greater at the groups that were added more amount of the lens cortex and nucleus material. CONCLUSIONS: it is important that we should not remain any lens cortex material remnant at cataract surgery.

Key Words: Aftercataract;In vitro capsular bag model;Lens epithelial cell culture;Lens fiber