| Cell Death of Corneal Fibroblasts Induced by Tumor Necrosis Factor-alphaand Interferon-gamma. |
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Yong Myeong Kim, Hyung Jin Koo, Eun Young Cho, Eui Sang Chung, Woo Jung Kim |
1Department of Ophthalmology, Samsung Medical Center, Sungkyunkwan University, School of Medicine, Seoul, Korea.
2Department of Ophthalmology, Kangwon National University College of Medicine, Chunchon, Korea. |
| Tumor necrosis factor - α 와 Interferon - γ 에 의해 유도된 각막섬유아세포의 세포사 |
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구형진(Hyung Jin Koo),정의상(Eui Sang Chung),김용명(Yong Myeong Kim),조은영(Eun Young Cho),김우중(Woo Jung Kim) |
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| Abstract |
PURPOSE
To evaluate the effect in the cell death of corneal fibroblasts when TNF-alphaand INF-gamma were given together. METHODS: Fibroblasts harvested from the human cornea were cultured in DMEM, then, nothing(control: Group 1), TNF-alphaonly(50 ng/ml : Group 2), INF-gammaonly(1.0 x 10(3)u/ml : Group 3), and a combination of both(Group 4) were added. We assessed the cell viability of the each group by the trypan blue exclusion assay at 4, 8, 12, 24, 48 hours after addition of cytokines. RESULTS: The cell viability at 48 hour after treatment was 94.27% in group 1, 90.68%(p=0.09) in group 2, 93.31%(p=0.45) in group3, and there was no statistical difference among the groups. Statistically signi-ficant decrease of the cell viability was achieved in group 4(82.86%, p=0.002). CONCLUSIONS: Cell death of human corneal fibroblasts had been increased after treatment with a combination of TNF-alphaand INF-gamma. These findings suggest that there could be some kind of interaction among the cytokines. |
| Key Words:
Cell death;Corneal fibroblasts;INF-gamma;TNF-alpha |
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