The Cellular Characteristics of the Secondary Pupillary Membrane.

Kang, Gu Min; Hur, Jong Wuk; Jang, Jin Ho; Lee, Sung Min; Kim, Joon Hyun; Ko, Myung Kyoo

Original Article

Journal of the Korean Ophthalmological Society 2002;43(5):823-829.
Published online May 1, 2002.

The Cellular Characteristics of the Secondary Pupillary Membrane.

Gu Min Kang, Jong Wuk Hur, Jin Ho Jang, Sung Min Lee, Joon Hyun Kim, Myung Kyoo Ko

Department of Ophthalmology, College of Medicine Hanyang University Hospital, Korea. fovea@hanyang.ac.kr

이차동공막의 세포학적 특성

강구민 ( Gu Min Kang ) , 허종욱 ( Jong Wuk Hur ) , 장진호 ( Jin Ho Jang ) , 이성민 ( Sung Min Lee ) , 김준현 ( Joon Hyun Kim ) , 고명규 ( Myung Kyoo Ko )

Abstract

PURPOSE
This study was performed to investigate the cellular characteristics of the secondary pupillary membrane. METHODS: The secondary pupillary membrane was removed from the anterior lens surface during cataract extraction from 2 patients with cataract associated with uveitis. Specimen from one patient was stained with hematoxylin-eosin with flat preparation method. Specimen from the other patient cultured for 1 and 2 weeks was observed with transmission electron microscopy. RESULTS: The flat preparation showed the neovascular membrane with pigment-laden cells. The cultured cells consisted of the well preserved vascular components which had the vascular endothelial cells and pericyte and pigment-laden cells lined by basement membrane on first week of culture. The iris pigment epithelial cell which contained the pigment granules within cytoplasm and lined by basement membrane were observed on second week of culture. CONCLUSIONS: These results suggest that the secondary pupillary membrane consists of vascular membrane and pigment epithelial cell of iris which is a major component of secondary pupillary membrane and secrets extracellular matrix.

Key Words: Culture;Flat preparation;Pigment epithelial cell;Secondary pupillary membrane;Ultrastructure