| The Effect of Transscleral Pressure on Human Scleral Thickness and Hydration. |
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Sang Bumm Lee, Jung Yoon Kwon, Henry F Edelhauser |
1Department of Ophthalmology, College of Medicine, Yeungnam University, Taegu, Korea, Korea. sbummlee@med.yu.ac.kr
2Department of Ophthalmology, School of Medicine, Kyungpook National University, Taegu, Korea.
3Emory Eye Center, Emory University, Atlanta, Georgia, USA. |
| 실험적 경공막 안약물 전달: 경공막압 증가에 따른 인체 공막의 두께와 수분함량의 변화 |
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이상범 ( Sang Bumm Lee ) , 권정윤 ( Jung Yoon Kwon ) , ( Henry F. Edelhauser ) |
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| Abstract |
PURPOSE
The purpose of this study was to evaluate the effect of transscleral pressure on the thickness and hydration of the human sclera. METHODS: Scleral sections excised from moist-chamber-stored human globes were mounted in a perfusion chamber that can create transscleral pressure. The scleral thickness was measured at 15-minute intervals using ultrasonic pachymetry with pressure changes of 15, 30, and 60 mmHg every 2 hours. The scleral hydration was measured by maintaining a constant pressure (15, 30, and 60 mmHg) for 4 hours, weighing the hydrated tissue, then drying for 24 hours at 120degrees C, and finally weighing the dry tissue. RESULTS: The changes of scleral thickness from the initial control thickness in the 7-mm perfusion chamber (n=6) were -0.38+/-2.11%, 0.26+/-2.99 %, and -3.92+/-3.40% at 15, 30, and 60 mmHg, respectively. In the 10-mm perfusion chamber (n=5), the thickness changes were -0.56+/-1.35%, -1.05+/-1.63%, and -2.71+/-1.60% at 15, 30, and 60 mmHg, respectively. Scleral thickness was slightly decreased with the increase of transscleral pressure. There was a statistically significant decrease in scleral thickness from 30 mmHg to 60 mmHg in the 7-mm perfusion chamber (p<0.05). Changes of the scleral hydration value in the 7-mm perfusion chamber were -0.79+/-1.06% (n=5), 0.66+/-1.40% (n=5), and 0.05+/-1 . 3 0 % (n=5) at 15, 30, and 60 mmHg, respectively. There were no statistically significant changes in the scleral hydration with transscleral pressure change of 0~60 mmHg (p>0.10). CONCLUSIONS: The scleral hydration remained essentially unchanged with the transscleral pressure change of 0 ~ 60 mmHg, whereas the scleral thickness showed a slight decrease with the increase of transscleral pressure. Further histologic studies will be needed to assess the ultrastructural change of human sclera in the future. |
| Key Words:
Human scleral hydration;Human scleral thickness;Transscleral ocular drug delivery;Transscleral pressure |
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