| Corneal Opacity after Repeated Photorefractive Keratectomy. |
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Chun Suk Jung, Ja Young Lee, Sung Kun Chung, Nam Ho Baek |
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Department of Ophthalmology, St. Mary`s Hostpital, College of Medicine, The Catholic University of Korea. |
| 반복된 굴절교정레이저각막절제술후 발생한 각막혼탁 |
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정천석(Chun Suk Jung),이자영(Ja Young Lee),정성근(Sung Kun Chung),백남호(Nam Ho Baek) |
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| Abstract |
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To study the histopathologic effects and possible collagen fiber alterations in corneal opacification after repeated photorefractive keratectomy(PRK) in human. PRK had been performed five times for 2 and 2/3 years using excimer laser. One year and ten months after last PRK, partial penetrating keratoplasty was performed. The cornea was stained with PAS, Alcian blue, Masson`s trichrome, Congo red. Oil red. O and stained immunohistochemically for collagens type III, IV, VI, VII. It was observed under light microscope(LM) and transmission electron microscope(TEM). LM findings showed thickening of epithelial layers, proliferation of subepithelial fibroblasts and absence of Bowman`s membrane. By TEM, disarray of collagen lamellae and electron dense deposits to adjacent keratocyte were observed. Histochemically, positive staining in Alcian blue, while negative in others. In immunohistochemistry, positive staining for type IV and VI collagens were noted. Corneal opacity after repeated PRK is due to deposits of type IV and VI collagens and acidic mucoprotein in extracelluar matrix, which is positive in Alcian blue stain. |
| Key Words:
Alcian blue stain;Collagen;Corneal opacity;Photorefractive keratectomy |
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