Glycogen Granules within the Co-cultured Conjunctival Epithelial Cells of Rabbit with Chlamydia Trachomatis.

Jun, BoklKwan; Ko, Myung Kyoo; Kim, Dong Seob

Original Article

Journal of the Korean Ophthalmological Society 1999;40(2):312-317.
Published online February 1, 1999.

Glycogen Granules within the Co-cultured Conjunctival Epithelial Cells of Rabbit with Chlamydia Trachomatis.

BoklKwan Jun, Myung Kyoo Ko, Dong Seob Kim

Department of Ophthalmology, College of Medicine, ahanyang University Hospital.

클라미디아와 동시배양된 토끼결막상피세포내의 당원과립

전복관(Bok Kwan Jun),고명규(Myung Kyoo Ko),김동섭(Dong Seob Kim)

Abstract

Inclusion bodies containing glycogen granules are characteristic feature of infected cells with Chlamydia trachomatis(C. trachomatis). In this study, we investigated the serial changes of the glycogen granules in a rabbit conjunctival epithelial cells co-cultivated with C. trachomatis.The epithelial cells isolated grom conjunctiva of rabbit were initially cultured for 3 weeks. After the cells had attained confluency, they were infected with C. trachomatis serotype D. After co-cultivation for 24, 48 and 96 hours, electronmicroscopic study was performed.After co-cultivation for 24 hours, a few glycogen granules were observed within the premature inclusion body containing some elementary bodies and reticulate bodies. After 48 hours of co-cultivation, typical inclusion bodies were observed and numerous electron-dense glycogen granules predominated around reticulate bodies. After 96 hours of co-cultivation, scattered glycogen granules were observed in granular pattern within the expanding inclusion occupying the bulk of the host cell cytoplasm.These results suggest that glycogen granules are closely related to the developmental cycle of the C. trachomatis and they may be an energy source for active multiplication of the reticulate bodies.

Key Words: Chlamysia trachomatis;Glycogen granule;Inclusion body