| Transfection of SV 40 Large T Antigen into Corneal Endothelial Cells. |
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Kyung Sun Cho, Timothy P Fleming, Eunjoo Hwang Lee, Choun Ki Joo |
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Department of Ophthalmology, kangnam St. Marys Hospital, The Catholic University Medical College. |
| 각막내피세포로의 SV 40 Large T 항원의 Transfection |
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조경선(Kyung Sun Cho) , (Timothy P Fleming) , 황은주(Eun Joo Hwang) , 주천기(Choun Ki Joo) |
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| Abstract |
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The coeneal endothelium is essential for the maintenance of normal corneal hydration, thickness, and transparency. However, corneal endothelial cells are incapable of significant proliferation in vivo. As we age, the density of corneal endothelial (CEN) cells gradually decreases. The goal of our study is to explore the possibility of enhancing the proliferation of corneal endothelial cells by introduction of SV 40 large T antigen, a transforming protein. To this end, introduction of protein into CEN cells was assessed by liposome assisted beta-galactosidase transfection in vivo, ex vivo, and in vivo. In all cases, cells treated with liposome-protein complex have shown dramatic blue stain in beta-galactosidase activity staining. This result convinced us that we could artificially introduce a foreign protein into a cell. To ascertain where SV 40 large T antigen is localized in the cell, purified SV 40 large T antigen was transfected into the cells using liposome and its presence was determined immunohistochemically. We show that the liposome delivered SV 40 large is localized in the nucleus and mitotic figures which may suggest its functional activity. |
| Key Words:
Corneal endothelial cells;beta-galactosidase;Liposome;SV 40 large T antigen;Transfection |
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