| The Expression of ICAM-1 by Cytokines and the Effect of Dexamethasone on ICAM-1 Expression in Cultured Keratocytes. |
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Ho Bum Kim, Hungwon Tchah |
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Department of Ophthalmology, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Korea. |
| 배양된 가토의 각막실질세포에서의 Cytokines 에 의한 ICAM - 1 의 발현과 Dexamethasone 에 의한 영향 |
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김호범(Ho Bum Kim),차홍원(Hung Won Tchah) |
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| Abstract |
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Intercellular adhesion molecule-1 (ICAM-1) Is a cell-surface glycoprotein that may regulate leukocyte-endothelial adhesion, leukocyte migration into the tissues, and leukocyte trafficking with target cells during inflammation and immune responses. Expression of ICAM-1 have been observed in diseased cornea, and it has been reported that expression on corneal cells is increased in the presence of pro-inflammatory cytokines. We investigated expression of ICAM-1 by various cytokines on cultured rabbit keratocytes and effect of dexamethasone on cytokine-induced ICAM 1 expression, using an ELISA technique. Cultured rabbit keratocytes were incubated for 24hrs with INF-gamma 10ng/ml, TNF-alpha 10ng/ml IL-1beta 5ng/ml, TGF beta 5ng/ml, with or without 0.1micromiter Dexamethasone. Rabbit keratocytes treated with cytokine or dexamethasone were incubated with antral-ICAM-1 for 15 hours. Expression of ICAM-1 was measured with ELISA technique. As a result, expression of ICAM-1 was increased in rabbit keratocytes stimulated with INF-gamma, TNF-alpha, TGF-beta, not IL-1beta, and dexamethasone inhibited expression of ICAM-1 in cells stimulated with INF-gamma, TNF-alpha. This results are helpful to understand the role of ICAM-1 in the pathophysiology of inflammatory corneal diseases and the action mechanism of glucocorticosteroids. Further study about expression of ICAM-1 and their regulation & modulation may lead to new therapies in treating inflammatory corneal diseases. |
| Key Words:
ICAM-1;Keratocyte;Cytokines;Dexamethasone;Immunocytochemical study |
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