Proliferative Effect of Retinal Glial Cells by Growth Factors.

Cho, Nam Chun; Lee, Min Cheol; Han, Hong Joo; Ahn, Byeong Gook

Original Article

Journal of the Korean Ophthalmological Society 1997;38(7):1179-1187.
Published online July 1, 1997.

Proliferative Effect of Retinal Glial Cells by Growth Factors.

Nam Chun Cho, Min Cheol Lee, Hong Joo Han, Byeong Gook Ahn

¹Department of Ophthalmology, Chonbuk National University, Medical School, Chonju.
²Department of Phathology, Chonnam National University, Medical School, Kwangju.

성장인자에 의한 망막 신경교세포의 증식능에 대한 연구

조남천(Nam Chun Cho),이민철(Min Cheol Lee),한홍주(Hong Joo Han),안병국(Byoung Cook Ahn)

Abstract

The authors tried to evaluate the effect of the growth factors on cell proliferation in the human retina astrocyte culture. Each growth factor, EGF, bFGF, GGF, IGF-1 or PDGF, together with BrdU was added to astrocytes-enriched cultures in the serum-free media. The proliferating effect of each growth factor was evaluated by a double immunofluo- rescenece labelling for GFAP and BrdU. By and addition of each growth factor, the number of retinal astrocytes at the synthetic stage significantly increased than those of control group (p<0.01). In comparision, PDGF was more potent than IGF-1 (p<0.01). Above data extended the concept of astrocytic proliferation caused by several growth factors in human retinal injury.

Key Words: Growth Factor;Astrocyte;Retinal Injury