| MIF Test Using Lens Protein as Antigen. |
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Hum Chung |
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Department of Ophthalmology, College of Medicine, Seoul National University, Seoul, Korea. |
| 수정체 단백에 (蛋白) 대한 MIF Test |
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정흠 ( Heum Chung ) |
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| Abstract |
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This experiment was performed to demonstrate that the cell-mediated hypersensitivity plays an important role in the development of the so-called endophthalmitis phaooanaphylactica. Fifteen albino guinea pigs were immunized with sclubte proteins of bovine lens with complete Freund's adjuvant. The immunizations were repeated 4 times at 5 days intervals. 5 days after the last injection, the skin test was performed with soluble lens protein, and liquid paraffin was injected into the peritoneal cavity. 7 days after paraffin injection, the peritoneal exudate cells were harvested, and were allowed to migrate on agar with and without antigen according to Salvin- Niahio method. All of them shows positive skin reaction and significant inhibition of migration under the presence of the antigen. |
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