| Effect of High Glucose on the Production of Reactive Oxygen Species in R28 Cells. |
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Jong Min Lee, Jae Woo Kim |
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Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr |
| R28세포에서 고포도당이 반응성산소종의 생성에 미치는 영향 |
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이종민ㆍ김재우 |
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Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea |
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| Abstract |
PURPOSE
To investigate the effect of high glucose (HG) on the production of reactive oxygen species (ROS) in retinal precursor R28 cells. METHODS: R28 cells were incubated with low glucose (5 mM) or HG (25 mM) for two days. Additionally, the cells were co-exposed to 50 micrometer N-acetyl cysteine or 100 micrometer L-arginine. Production of nitric oxide (NO), ROS, and superoxide were assessed by Griess assay, DCFH-DA assay, and modified cytochrome c assay, respectively. RESULTS: HG increased the production of NO, ROS, and superoxide, which were abolished by antioxidants NAC and L-arginine (a substrate for NO production). CONCLUSIONS: HG increased ROS production in R28 cells. Thus, HG may cause cellular dysfunction and damage by inducing oxidative stress in retinal ganglion cells. |
| Key Words:
High glucose;Oxidative stress;Reactive oxygen species;Retinal ganglion cells |
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