Effect of Trypan Blue on the Survival of Cultured Trabecular Meshwork Cells. |
Hye Ri Kang, Seok Jin Hwang, Jae Woo Kim |
Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr |
트리판 블루가 배양된 섬유주세포의 생존에 미치는 영향 |
강혜리⋅황석진⋅김재우 |
대구가톨릭대학교 의과대학 안과학교실 |
Correspondence:
Jae Woo Kim, Email: jwkim@cu.ac.kr |
Received: 28 July 2016 • Revised: 17 August 2016 • Accepted: 31 January 2017 |
Abstract |
PURPOSE To evaluate the effects of trypan blue (TB) on the survival of cultured human trabecular meshwork cells (HTMCs). METHODS: Primarily cultured HTMCs were exposed to 0.05, 0.10 or 0.50% TB for 1, 5 or 30 min. Cellular survival was assessed using the MTT assay and degree of apoptosis was analyzed with flow cytometry using annexin-V/propidium iodide double staining. RESULTS: Long-term exposure or high concentration of TB decreased the survival of HTMCs (p < 0.05). In flow cytometric analysis, exposure to 0.50% TB for 30 min increased the degree of apoptosis (p < 0.05). Commercial TB decreased cell survival after exposure for 5 min and increased the degree of apoptosis after exposure for 30 min (p < 0.05). CONCLUSIONS: TB may cause cellular damage of cultured HTMCs and apoptosis could be the underlying mechanism. In TB-assisted cataract surgery, TB should be used for the shortest time possible and removed completely. |
Key Words:
Apoptosis;Flow cytometry;Survival;Trabecular meshwork cell;Trypan blue |