-
Original Article
-
J Korean Ophthalmol Soc. 2018;59(6):497-504. Published online June 15, 2018.
DOI: https://doi.org/10.3341/jkos.2018.59.6.497
- Xenotransplantation of Cultured Human Corneal Endothelial Cell Sheets.
-
Yoon Pyo Lee, Tae Young Chung, Joon Young Hyon, Young Joo Shin
-
Department of Ophthalmology, Kangnam Sacred Heart Hospital, Hallym University College of Medicine, Seoul, Korea. schinn@daum.net
Department of Ophthalmology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
Department of Ophthalmology, Seoul National University Bundang Hospital, Seongnam, Korea.
- 배양사람각막내피세포판의 이종이식
- 이윤표<sup>1</sup>⋅정태영<sup>2</sup>⋅현준영<sup>3</sup>⋅신영주<sup>1</sup>
- Department of Ophthalmology, Kangnam Sacred Heart Hospital, Hallym University College of Medicine, Seoul, Korea. schinn@daum.net
Department of Ophthalmology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
Department of Ophthalmology, Seoul National University Bundang Hospital, Seongnam, Korea.
- Corresponding author: Young Joo Shin ,Email: schinn@daum.net
- Received: February 22, 2018; Revised: April 16, 2018 Accepted: June 4, 2018.
- Abstract
- PURPOSE
To investigate the possibility of transplantation into rabbits of collagenase-induced cultured human corneal endothelial cell plate-rabbit corneal stromal complexes. METHODS: Human corneal endothelial cells were isolated from residual corneal limbus samples and treated with collagenase to create corneal endothelial cell sheets. Cultured sheets were transplanted into the rabbit stroma after the rabbit corneal endothelial cells and Descemet's membrane were removed. Hematoxylin-and-eosin staining and immunofluorescence staining for collagen VIIIa2 (COL8A2) and zonula occludens-1 (ZO-1) were performed. The cultured human corneal endothelial cell sheet-rabbit corneal stromal complex was transplanted into rabbits. On days 3, 5, and 7, the transplanted corneas were photographed and corneal opacity was measured. One week later, the rabbits were sacrificed. Hematoxylin-and-eosin staining and immunofluorescence staining for COL8A2 were performed. RESULTS: The cultured cells were immunofluorescently stained for collagen VIIIa1 and ZO-1. Collagenase-treated cultured human corneal endothelial cells formed monolayers on day 7 after transplantation into the rabbit corneal stroma and immunofluorescently stained for COL8A2 and ZO-1. The cultured human corneal endothelial cell sheet-rabbit stroma complex transplanted into rabbits was transparent on days 3 and 5, but corneal opacity developed by day 7. Histologic examination revealed 3,3′-dioctadecyloxacarbocyanine perchlorate (DIO)-stained corneal endothelium (green) and hard-tissue lymphocytes had infiltrated the cultured corneal endothelial cell plate-rabbit corneal stromal complex graft group. CONCLUSIONS: The cultured corneal endothelial cell sheet-rabbit corneal stromal complex prepared with the aid of collagenase showed a potential method for corneal endothelial cell transplantation.
Keywords :Collagenase;Corneal endothelial cell;Corneal transplantation;Cultured human corneal endothelial cell sheet;Endothelial cell transplantation
