J Korean Ophthalmol Soc > Volume 52(6); 2011 > Article
Journal of the Korean Ophthalmological Society 2011;52(6):671-678.
DOI: https://doi.org/10.3341/jkos.2011.52.6.671    Published online June 15, 2011.
Effects of Mitomycin C on Scleral Collagen Fibrils According to Atomic Force Microscopy.
Hui Jae Lee, Samjin Choi, Youjin Cheong, Gyeong Bok Jung, Kyung Hyun Jin, Hun Kuk Park, Seung Jun Lee
1Department of Ophthalmology, School of Medicine, Kangwon National University, Chuncheon, Korea. opticus@kangwon.ac.kr
2Department of Biomedical Engineering, College of Medicine, Kyung Hee University, Seoul, Korea.
3Department of Ophthalmology, College of Medicine, Kyung Hee University, Seoul, Korea.
4Program of Medical Engineering, Kyung Hee University, Seoul, Korea.
원자현미경을 이용한 마이토마이신 노출 시간에 따른 공막 표면 콜라젠의 변화 관찰
이희재1⋅최삼진2⋅정유진2⋅정경복2⋅진경현3⋅박헌국2,4⋅이승준1
Department of Ophthalmology, School of Medicine, Kangwon National University1, Chuncheon, Korea Departments of Biomedical Engineering2, Ophthalmology, College of Medicine3, Program of Medical Engineering, Kyung Hee University4, Seoul, Korea
Abstract
PURPOSE
To investigate the effects of mitomycin C on the scleral collagen surfaces using atomic force microscopy (AFM). METHODS: Two non-contact mode AFM machines were used to observe changes in the morphological characteristics of human scleral surfaces before and after one, three, and five minutes of 0.02% mitomycin C application. Based on AFM topography and deflection images of the collagen fibril, the morphological characteristics of scleral fibrils including the fibril diameter and D-period were measured using the line profile. RESULTS: The sclera collagen fibril treated with 0.02% mitomycin C for one minute did not show any significant increases in mean fibril diameter (155.04 +/- 17.46 nm) or mean D-periodicity (70.02 +/- 3.33 nm), compared to those of the control group. However, the scleral collagen fibrils treated with 0.02% mitomycin C for three and five minutes showed significant increases in mean fibril diameter (182.33 +/- 16.33 nm, 199.20 +/- 12.40 nm, respectively) and mean D-periodicity (70.27 +/- 13.66 nm, 72.75 +/- 19.32 nm, respectively), compared to those of the control group. CONCLUSIONS: The present study examined the structural changes in the scleral collagen fibrils before and after mitomycin C application according to atomic force microscopy. The results indirectly suggest that three or more minutes of 0.02% mitomycin C application affects the morphology of scleral collagen.
Key Words: Atomic force microscopy;Collagen fibrils;Diameter;D-banding;Human sclera;Mitomycin C


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