Histologic Study in Transplantation of Cultured Rabbit Retinal Pigment Epithelium.

Kwak, Hyung Woo; Lee, Jae Hyun

Original Article

Journal of the Korean Ophthalmological Society 1997;38(3):391-397.
Published online March 1, 1997.

Histologic Study in Transplantation of Cultured Rabbit Retinal Pigment Epithelium.

Hyung Woo Kwak, Jae Hyun Lee

Department of Ophthalmology, College of Medicine, Kyunghee University, Seoul, Korea.

배양 망막색소상피 세포 이식 후 발생된 조직학적 변화에 대한 연구

곽형우(Hyung Woo Kwak),이재현(Jae Hyun Lee)

Abstract

The authors investigated the possibility of transplantion of cultured retinal pigment epithelial cell to normal pigmented rabbit retina. Focal retinal detachment was made in the pigment rabbit retina, and the cultured RPE cells were injected into subretinal space. The neural retina spontaneously rettached withim 6 days. At 4 weeks after tranplantation, eyes were enucleated and examined with light-microscopy and electron-microscopy. The transplanted RPE cells were proliferated with multilayer in electronmicroscopic finding, and the tight-junction was found between proliferative RPE cells. The outer segment and nucleus of photoreceptor cell were well preserved in microscopic finding. As a result, the cultured RPE cells can be sucessfully transplanted to normal pigmented rabbit retina, and photoreceptor cell was not changed after transplantation.

Key Words: Cultured RPE (retinal pigment epithelium) cell;Pigmented rabbit retina;Retinal pigment epithelium cell transplantation