| Effect of Valproic Acid on Nitric Oxide and Nitric Oxide Synthase in Trabecular Meshwork Cell. |
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Myung Seo Son, Cheul Ho Park, Jae Woo Kim |
1Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr
2Hyundai Eye Clinic, Kumi, Korea. |
| 섬유주세포에서 발프로익산이 일산화질소의 생성과 일산화질소합성효소의 발현에 미치는 영향 |
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손명서1⋅박철호2⋅김재우1 |
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대구가톨릭대학교 의과대학 안과학교실1, 현대안과의원2 |
Correspondence:
Jae Woo Kim,
Email: jwkim@cu.ac.kr
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Received: 6 July 2017 • Revised: 16 August 2017 • Accepted: 31 May 2018 |
| Abstract |
PURPOSE
To investigate the effects of valproic acid on the production of nitric oxide (NO) and expression of endothelial nitric oxide synthase (eNOS) in cultured human trabecular meshwork cells (HTMC). METHODS: Primarily cultured HTMC were exposed to 0.25, 0.5, and 1.0 mM valproic acid for 6, 12, and 24 hours. Expression of eNOS mRNA was assessed with Reverse transcription-polymerase chain reaction, and production of NO was assessed with Griess assay. Cellular survival was assessed with the 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay. RESULTS: Valproic acid at concentrations of 0.25, 0.5, 1.0 mM did not affect the cellular survival of HTMC significantly after exposure for 24 hours. Valproic acid increased NO production in a dose- and time-dependent manner. Also, valproic acid increased the degree of eNOS mRNA expression in a dose-dependent manner in HTMC. CONCLUSIONS: Valproic acid increases production of NO and expression of eNOS mRNA in HTMC. Thus, valproic acid might increase aqueous outflow through the trabecular meshwork. |
| Key Words:
Endothelial nitric oxide synthase (eNOS);Nitric oxide;Trabecular meshwork;Valproic acid |
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