| Comparison of the Toxicity of Olopatadine Anti-allergic Ophthalmic Agents on Rabbit Conjunctival Cells. |
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Young Hwan Jeong, Su Gyeong Jang, Jong Soo Lee |
1Department of Ophthalmology, Pusan National University College of Medicine, Busan, Korea. jongsool@pusan.ac.kr
2BioMedical Research Institute of Pusan National University Hospital, Busan, Korea. |
| 올로파타딘이 토끼 결막세포에 미치는 독성 비교 |
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정영환1 · 장수경1,2 · 이종수1,2 |
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부산대학교 의과대학 안과학교실1, 부산대학교병원 의생명연구원2 |
Correspondence:
Jong Soo Lee,
Email: jongsool@pusan.ac.kr
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Received: 26 July 2019 • Revised: 27 August 2019 • Accepted: 6 December 2019 |
| Abstract |
PURPOSE
To compare the in vitro toxicity of commercial olopatadine anti-allergic ophthalmic agents on cultured rabbit's conjunctival cells according to concentrations and exposure times. METHODS: Rabbit conjunctival cells were exposed to anti-allergic olopatadine ophthalmic agents, (Patanol® [0.1% olopatadine hydrochloride; Alcon, Fort Worth, TX, USA], Pataday® [0.2% olopatadine hydrochloride; Alcon], and Pazeo® [0.7% olopatadine hydrochloride; Alcon]) at concentrations of 5%, 10%, and 15% for periods of 30 minutes, 1, 2, 3, and 6 hours, respectively. Cell proliferation and injury assays were performed using the methylthiazoltetrazolium and lactate dehydrogenase (LDH) leakage assays. We checked the composition of the three anti-allergic agents, and performed light and transmission electron microscopy to compare the morphological changes in cells. RESULTS: The conjunctival cell proliferation was inhibited after 1 hour exposure to each olopatadine ophthalmic agent, with significant cell proliferation inhibited using 15% of each drug. The proliferation of conjunctival cells was inhibited during 6 hours of drug exposure at all concentrations of Pataday® and Pazeo®. The titer of LDH increased from 3 hours after drug exposure, but 15% Pazeo® significantly increased the LDH titer at 2 hours after drug exposure. As the concentration of the drug increased, the LDH titer also significantly increased. The cellular morphological changes of conjunctival cells were in the increasing order of Pazeo®, Pataday®, and Patanol® with a high concentration of olopatadine hydrochloride. CONCLUSIONS: Among the anti-allergic olopatidine ophthalmic agents, higher olopatadine concentrations in the increasing order of Pazeo®, Pataday®, and Patanol® resulted in cytoplasmic damage of conjunctival cells, but there was no severe damage to the cytoplasmic or the nuclear membranes. |
| Key Words:
Allergy;Conjunctivitis;Olopatadine hydrochloride;Toxicity |
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