| Effects of Hydrogen Sulfide and Nitric Oxide on the Permeability of Cultured Trabecular Meshwork Cells. |
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Myung Seo Son, Min Ju Baek, Jae Woo Kim |
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Department of Ophthalmology, Daegu Catholic University College of Medicine, Daegu, Korea. jwkim@cu.ac.kr |
| 황화수소가 섬유주단층세포층 투과도 증가에 미치는 영향과 일산화질소의 상호작용에 관한 연구 |
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손명서⋅백민주⋅김재우 |
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대구가톨릭대학교 의과대학 안과학교실 |
Correspondence:
Jae Woo Kim,
Email: jwkim@cu.ac.kr
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Received: 4 April 2019 • Revised: 20 August 2019 • Accepted: 21 February 2020 |
| Abstract |
PURPOSE
To investigate the effects of hydrogen sulfide (H₂S) on the permeability of a cultured human trabecular meshwork cells (HTMC) monolayer and its interaction with nitric oxide (NO). METHODS: After exposing primary cultured HTMCs to 0, 50, 100, and 500 µM sodium hydrogen sulfide (NaHS) for 6 hours, the permeabilities through the HTMC monolayer were measured using a Transwell assay with carboxyfluorescein. The production of NO and eNOS mRNA expression were assessed using the Griess assay and reverse transcription-polymerase chain reaction, respectively. In addition, 0, 1, and 10 µM NaHS and 10 µM sodium nitroprusside (SN) were co-exposed to evaluate the possible synergistic effect of H₂S and NO. RESULTS: Greater than 100 µM NaHS increased the permeability through the HTMC monolayer in a dose-dependent manner (p < 0.05). These increased permeabilities were not accompanied by NO production or eNOS mRNA expression (p > 0.05). When 0, 1, and 10 µM NaHS and 10 µM SN were exposed together, there was no significant change of permeability, NO production, or eNOS mRNA expression (all, p > 0.05). CONCLUSIONS: NaHS at high concentrations increased the permeability of the HTMC monolayer, which was not affected by NO. NaHS at low concentrations did not show a synergistic effect with NO. Thus, H₂S at high concentrations may increase trabecular outflow, which may not be associated with NO. |
| Key Words:
Hydrogen sulfide;Nitric oxide;Permeability;Trabecular meshwork |
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