J Korean Ophthalmol Soc > Volume 58(3); 2017 > Article
Journal of the Korean Ophthalmological Society 2017;58(3):327-332.
DOI: https://doi.org/10.3341/jkos.2017.58.3.327    Published online March 15, 2017.
Effect of Trypan Blue on the Survival of Cultured Trabecular Meshwork Cells.
Hye Ri Kang, Seok Jin Hwang, Jae Woo Kim
Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr
트리판 블루가 배양된 섬유주세포의 생존에 미치는 영향
강혜리⋅황석진⋅김재우
대구가톨릭대학교 의과대학 안과학교실
Correspondence:  Jae Woo Kim,
Email: jwkim@cu.ac.kr
Received: 28 July 2016   • Revised: 17 August 2016   • Accepted: 31 January 2017
Abstract
PURPOSE
To evaluate the effects of trypan blue (TB) on the survival of cultured human trabecular meshwork cells (HTMCs). METHODS: Primarily cultured HTMCs were exposed to 0.05, 0.10 or 0.50% TB for 1, 5 or 30 min. Cellular survival was assessed using the MTT assay and degree of apoptosis was analyzed with flow cytometry using annexin-V/propidium iodide double staining. RESULTS: Long-term exposure or high concentration of TB decreased the survival of HTMCs (p < 0.05). In flow cytometric analysis, exposure to 0.50% TB for 30 min increased the degree of apoptosis (p < 0.05). Commercial TB decreased cell survival after exposure for 5 min and increased the degree of apoptosis after exposure for 30 min (p < 0.05). CONCLUSIONS: TB may cause cellular damage of cultured HTMCs and apoptosis could be the underlying mechanism. In TB-assisted cataract surgery, TB should be used for the shortest time possible and removed completely.
Key Words: Apoptosis;Flow cytometry;Survival;Trabecular meshwork cell;Trypan blue


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