| Effect of Bevacizumab and Ranibizumab on the Expression of eNOS in Trabecular Meshwork Cells. |
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Se Eun Kim, Jae Woo Kim |
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Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr |
| 섬유주세포에서 베바시주맙과 라니비주맙이 일산화질소합성효소의 발현에 미치는 영향 |
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김세은⋅김재우 |
Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea
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| Abstract |
PURPOSE
To compare the effects of bevacizumab and ranibizumab on the expression of eNOS in cultured human trabecular meshwork cells (HTMC). METHODS: Primarily cultured HTMC were exposed to 0.5 mg/mL bevacizumab and ranibizumab using 1% serum-containing media for 30 minutes. Expression of eNOS mRNA was assessed with RT-PCR. Additionally, after exposure to 20 ng/mL of vascular endothelial growth factor (VEGF) and 0.5 mg/mL bevacizumab and ranibizumab, the production of nitric oxide was assessed with Griess assay. RESULTS: VEGF increased the production of nitric oxide in HTMC. Bevacizumab and ranibizumab decreased the expression of eNOS mRNA and production of nitric oxide (p < 0.05) in HTMC. The decrease in eNOS mRNA expression and production of nitric oxide was not significant between bevacizumab and ranibizumab (p > 0.05). CONCLUSIONS: In HTMC, both bevacizumab and ranibizumab decreased the expression of eNOS mRNA with no significant difference observed between the two drugs. |
| Key Words:
Bevacizumab;eNOS;Nitric oxide;Ranibizumab;Trabecular meshwork |
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