J Korean Ophthalmol Soc > Volume 54(3); 2013 > Article
Journal of the Korean Ophthalmological Society 2013;54(3):490-495.
DOI: https://doi.org/10.3341/jkos.2013.54.3.490    Published online March 15, 2013.
Effect of Ascorbic Acid Against the Oxidative Stress-Induced Cellular Senescence in Trabecular Meshwork Cells.
Jae Woo Kim, Sun Hee Kang, Keun Woo Lee
Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr
섬유주세포에서 아스코르빈산이 산화스트레스에 대한 세포노화에 미치는 영향
김재우⋅강선희⋅이근우
Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea
Abstract
PURPOSE
To investigate the effect of L-ascorbic acid (LAA) on oxidative-stress-induced cellular senescence in trabecular meshwork (TM) cells. METHODS: Primarily cultured human TM cells were exposed to 0, 10, or 100 microM hydrogen peroxide for 7 days with or without co-exposure of LAA. Cellular survival and nitrite production were assessed with MTT and Griess assays, respectively. SA-beta-gal staining was performed to quantify cellular senescence. RESULTS: Hydrogen peroxide decreased cellular survival, accompanied by decreased nitric oxide (NO) production. These decreases of cellular survival and NO production were abolished by co-exposure of 100 microM LAA. Analysis of SA-beta-gal staining revealed that LAA inhibited hydrogen peroxide-induced cellular senescence by 6.8% (p < 0.05). CONCLUSIONS: LAA may have a protective effect against the oxidative-stress-induced cellular senescence in TM cells.
Key Words: Ascorbic acid;Oxidative stress;Senescence;Trabecular meshwork cells


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