Analysis of CCL5 Concentration in Tears of Dry Eye Patients. |
Won Choi, Seung Hyun Lee, Kyung Chul Yoon |
Department of Ophthalmology, Chonnam National University Hospital, Chonnam National University Medical School, Gwangju, Korea. kcyoon@chonnam.ac.kr |
건성안 환자의 눈물 내 케모카인 리간드 5의 농도 분석 |
최 원⋅이승현⋅윤경철 |
Department of Ophthalmology, Chonnam National University Hospital, Chonnam National University Medical School, Gwangju, Korea |
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Abstract |
PURPOSE To investigate the expression of CCL5/RANTES (regulated upon activation, normal T cell expressed and secreted) in the tears of dry eye patients. METHODS: Forty patients with dry eye (15 Sjogren's and 25 non-Sjogren's syndrome patients) and ten control subjects were recruited for the present study. The concentration of RANTES in tears was measured using an enzyme-linked immunosorbent assay. The correlations between RANTES level, tear film and ocular surface parameters, including tear film break-up time, basal tear secretion, tear clearance rate, corneal sensation, keratoepitheliopathy, and conjunctival goblet cell density, were analyzed in patients with dry eye syndrome. RESULTS: The concentrations of RANTES were 435.46 +/- 104.45 pg/ml in Sjogren's syndrome patients, 257.42 +/- 46.72 pg/ml in non-Sjogren's syndrome patients, and 97.53 +/- 29.15 pg/ml in the control patients (p < 0.01). The levels correlated significantly with basal tear secretion, tear clearance rate, keratoepitheliopathy, and goblet cell density (p < 0.05). CONCLUSIONS: CCL5/RANTES level increases in the tears of dry eye patients and correlates with various tear film and ocular surface parameters. |
Key Words:
CCL5/RANTES;Dry eye;Ocular surface;Sjogren's syndrome;Tear film |
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