Autophagy of Human Tenon's Capsule Fibroblasts Induced by Mitomycin-C.

Park, Channy; Park, Raekil; Ryu, Seong Kwang; Lee, Dong Wook

doi:2011.52.11.1337

J Korean Ophthalmol Soc > Volume 52(11); 2011 > Article

Original Article

Journal of the Korean Ophthalmological Society 2011;52(11):1337-1343.
DOI: https://doi.org/10.3341/jkos.2011.52.11.1337 Published online November 15, 2011.

Autophagy of Human Tenon's Capsule Fibroblasts Induced by Mitomycin-C.

Channy Park, Raekil Park, Seong Kwang Ryu, Dong Wook Lee

¹Department of Audiology, Nambu University, Gwangju, Korea.
²VestibuloCochlear Research Center & Department of Microbiology, Wonkwang University, Iksan, Korea.
³Department of Ophthalmology, Chonbuk National University Medical School, Jeonju, Korea. ldw@jbnu.ac.kr

마이토마이신씨에 의해 유도된 인간 테논낭 섬유아세포의 자가포식현상

박찬희^1,2⋅박래길^1,2⋅유성광³⋅이동욱³

Department of Audiology, Nambu University¹, Gwangju, Korea VestibuloCochlear Research Center & Department of Microbiology, Wonkwang University², Iksan, Korea Department of Ophthalmology, Chonbuk National University Medical School³, Jeonju, Korea

Abstract

PURPOSE
The present study investigated whether an autophagic process is involved in the apoptotic death of human tenon's capsule fibroblasts (HTCFs) caused by mitomycin-C. METHODS: An autophagic phenotype was tested using fluorescence microscopy and flow cytometry with specific biological staining dyes including monodansylcadaverine and acridine orange and microtubule-associated protein 1 light chain 3 (LC3). RESULTS: Treatment with mitomycin-C (0.4 mg/ml) increased the acidic vesicular organelles of tenon's capsule fibroblasts in a time dependent manner. Mitomycin-C induced both LC3-II cleavage and beclin-1 expression. 3-MA, a pharmacological inhibitor of autophagy, inhibited the mitomycin-C induced increase of acidic vesicular organelleS. CONCLUSIONS: Autophagy was induced with 0.4 mg/ml mitomycin-C in tenon's capsule fibroblasts. And, autophagic mechanisms may be involved in the early stage of apoptosis of fibroblasts.

Key Words: Autophagy;Fibroblast;Mitomycin-C