J Korean Ophthalmol Soc > Volume 53(3); 2012 > Article
Journal of the Korean Ophthalmological Society 2012;53(3):466-472.
DOI: https://doi.org/10.3341/jkos.2012.53.3.466    Published online March 15, 2012.
Effects of Cyclosporin on Pterygium Fibroblasts.
Jong Soo Lee, Seung Wook Lee, Sang Jun Lee, Na Mi Kim
1Department of Ophthalmology, Pusan National University College of Medicine, Busan, Korea. jongsool@pusan.ac.kr
2Department of of Ophthalmology, Kosin University College of Medicine, Busan, Korea.
익상편조직의 섬유모세포에 미치는 사이클로스포린의 영향
이종수1⋅이승욱2⋅이상준1⋅김나미1
Department of Ophthalmology, Pusan National University College of Medicine1, Busan, Korea Department of of Ophthalmology, Kosin University College of Medicine2, Busan, Korea
Abstract
PURPOSE
To evaluate the response and cellular damage of cultured human pterygial cells according to the concentration and exposure time of topical cyclosporin. METHODS: Human pterygial cells were exposed to a cyclosporin A concentrations of 0.1 microg/ml (0.0001%), 1 microg/ml (0.0001%), 10 microg/ml (0.001%), 100 microg/ml (0.01%), or 500 microg/ml (0.05%) for 5 or 10 minutes. An MTT-based colorimetric assay was performed to assess the metabolic activity of cellular proliferation, and a lactate dehydrogenase (LDH) leakage assay was used to determine cellular damage. The extra-cellular matrix of PIP, laminin and MMP were evaluated, and the measurement of pro-inflammatory cytokine, TNF-alpha and IL-1b. IL-6, IL-8 was performed using ELISA kits. RESULTS: The pterygial cellular inhibitory effect of cyclosporin was similar to that of the control according to the concentration and exposure time (p > 0.05). Compared with the control, the level of LDH did not show a statistically significant difference between concentration and exposure time (p > 0.05). There was no significant difference of inhibitory effects by PIP, laminin, or MMP between the experimental and control groups (p > 0.05). The production of TNF-alpha and IL from the experimental pterygial cells due to the effect of cyclosporin was not significantly different from that of the control at a longer exposure time or stronger concentration (p > 0.05). CONCLUSIONS: The response of pterygial cells to topical cyclosporin A at concentrations less than 0.05% for less than 10 minutes of exposure time showed no prevention of pterygial recurrence. With regard to cellular damage, little effects on inhibition of PIP, laminin, MMP, IL, and TNF-alpha were observed compared with that of the control.
Key Words: Cyclosporin;IL;LDH;MMP;Pterygium


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