Effects of Cyclosporin on Pterygium Fibroblasts. |
Jong Soo Lee, Seung Wook Lee, Sang Jun Lee, Na Mi Kim |
1Department of Ophthalmology, Pusan National University College of Medicine, Busan, Korea. jongsool@pusan.ac.kr 2Department of of Ophthalmology, Kosin University College of Medicine, Busan, Korea. |
익상편조직의 섬유모세포에 미치는 사이클로스포린의 영향 |
이종수1⋅이승욱2⋅이상준1⋅김나미1 |
Department of Ophthalmology, Pusan National University College of Medicine1, Busan, Korea Department of of Ophthalmology, Kosin University College of Medicine2, Busan, Korea |
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Abstract |
PURPOSE To evaluate the response and cellular damage of cultured human pterygial cells according to the concentration and exposure time of topical cyclosporin. METHODS: Human pterygial cells were exposed to a cyclosporin A concentrations of 0.1 microg/ml (0.0001%), 1 microg/ml (0.0001%), 10 microg/ml (0.001%), 100 microg/ml (0.01%), or 500 microg/ml (0.05%) for 5 or 10 minutes. An MTT-based colorimetric assay was performed to assess the metabolic activity of cellular proliferation, and a lactate dehydrogenase (LDH) leakage assay was used to determine cellular damage. The extra-cellular matrix of PIP, laminin and MMP were evaluated, and the measurement of pro-inflammatory cytokine, TNF-alpha and IL-1b. IL-6, IL-8 was performed using ELISA kits. RESULTS: The pterygial cellular inhibitory effect of cyclosporin was similar to that of the control according to the concentration and exposure time (p > 0.05). Compared with the control, the level of LDH did not show a statistically significant difference between concentration and exposure time (p > 0.05). There was no significant difference of inhibitory effects by PIP, laminin, or MMP between the experimental and control groups (p > 0.05). The production of TNF-alpha and IL from the experimental pterygial cells due to the effect of cyclosporin was not significantly different from that of the control at a longer exposure time or stronger concentration (p > 0.05). CONCLUSIONS: The response of pterygial cells to topical cyclosporin A at concentrations less than 0.05% for less than 10 minutes of exposure time showed no prevention of pterygial recurrence. With regard to cellular damage, little effects on inhibition of PIP, laminin, MMP, IL, and TNF-alpha were observed compared with that of the control. |
Key Words:
Cyclosporin;IL;LDH;MMP;Pterygium |
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