J Korean Ophthalmol Soc > Volume 49(12); 2008 > Article
Journal of the Korean Ophthalmological Society 2008;49(12):1981-1988.
DOI: https://doi.org/10.3341/jkos.2008.49.12.1981    Published online December 15, 2008.
The Effectiveness of Tranilast in the Prevention of Posterior Capsular Opacity.
Ho Young Kim, Hyeon Il Lee, Yeoun Sook Chun, Jae Chan Kim
1Department of Ophthalmology, Chung-Ang University College of Medicine, Seoul, Korea.
2GM Eye Clinic, Seoul, Korea. fineview21@empal.com
백내장 수술 후 후낭혼탁 발생 억제에 대한 트라닐라스트의 유용성
김호영1ㆍ이헌일2ㆍ전연숙1ㆍ김재찬1
Department of Ophthalmology, Chung-Ang University College of Medicine1, Seoul, Korea / GM Eye Clinic2, Seoul, Korea
Abstract
PURPOSE
To evaluate the inhibitory effect of tranilast on the formation of posterior capsular opacity (PCO) after a cataract operation ex vivo and in a rabbit model. METHODS: A human lens epithelial cell line (B3) was treated with 0.005-0.1 mM tranilast. Cytotoxicity assessment and effective dosage determination of tranilast were performed using MTT assays. B3 cell lines were cultured in Eagle's minimal essential medium (EMEM) containing 20% FBS with different concentrationsof tranilast, and morphological differences were observed. To investigate the effect of tranilast on cytokine production in B3 cell lines, cells were treated with 0.01 mM tranilast and expression profiles of cytokines were analyzed by RT-PCR. After performing phacoemulsification and intraocular lens implantation in 10 white rabbits, 0.5% tranilast eye drops were given 4 times per day, and the severity of PCO was evaluated bi-weekly using POCOman for 8 weeks after the operation. RESULTS: Cell death was observed in the 0.05 mM tranilast-treated B3 cell lines, and inhibition of epithelial-mesenchymal transition (EMT) was also observed in the 0.01 mM tranilast-treated B3 cell lines. TGF-beta1/2, IL-18, and CDK7 mRNA expression decreased in the 0.01 mM tranilast-treated B3 cell lines. Significant suppression of PCO formation was observed in rabbits treated with 0.5% tranilast eye drops 5 weeks post operative (p<0.05). CONCLUSIONS: The results from this study show that tranilast suppresses EMT through inhibition of TGF-beta, IL-18,and CDK7 expression. The results suggest that tranilast can be used toprevent PCO formation after cataract surgery.
Key Words: Cytokine;Epithelial-mesenchymal transition;Lens epithelial cell lines;Posterior capsular opacity;Tranilast


ABOUT
BROWSE ARTICLES
EDITORIAL POLICY
FOR CONTRIBUTORS
Editorial Office
SKY 1004 Building #701
50-1 Jungnim-ro, Jung-gu, Seoul 04508, Korea
Tel: +82-2-583-6520    Fax: +82-2-583-6521    E-mail: kos08@ophthalmology.org                

Copyright © 2024 by Korean Ophthalmological Society.

Developed in M2PI

Close layer
prev next