| Effects of Oxidative Stress and Antioxidant on the Expression of Heme Oxygenase-1 in Human RPE. |
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Young Jun Kim, Hee Seung Chin |
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Department of Ophthalmology, Inha University School of Medicine, Incheon, Korea. hschin@inha.ac.kr |
| 인체망막색소상피세포에서 헴산화효소-1의 발현에 대한 산화스트레스 및 항산화물질의 영향 |
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김영준ㆍ진희승 |
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Department of Ophthalmology, Inha University School of Medicine, Incheon, Korea |
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| Abstract |
PURPOSE
To evaluate the effects of oxidative stress and antioxidantson heme oxygenase-1 (HO-1) in cultured human retinal pigment epithelial (RPE) cells. METHODS: Cultured RPE cells were challenged with different concentrations of hydrogen peroxide (H2O2), and the HO-1 mRNA level was determined by RT-PCR after 24 hours and 48 hours of incubation independently. Additionally, the HO-1 mRNA level was measured after preincubating RPE cells with N-acetylcystein (NAC) as the antioxidant for 30 minutes and then challenging the cells with H2O2. RESULTS: The expression of the HO-1 mRNA level increased after H2O2 exposure, and this level was proportional to the increased H2O2 concentration (p<0.05). Expression of the HO-1 mRNA level decreased when the RPE cells were preincubated with NAC (p<0.05). CONCLUSIONS: In human RPE cells, the HO-1 level increased in proportion to the degree of oxidative stress and decreased with exposure to antioxidants. Expression of HO-1 may be helpful in preventing retinal disease, which occurs due to oxidative stresses such as age-related macular degeneration. |
| Key Words:
Age related macular degeneration;Antioxidant;Heme oxygenase-1;Oxidative stress;Retinal pigment epithelium |
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