| Effect of Dexamethasone on the Production of Nitric Oxide in Trabecular Meshwork Cells. |
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Soo Yoon Lee, Jae Woo Kim |
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Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea. jwkim@cu.ac.kr |
| 섬유주세포에서 덱사메타존이 산화질소의 생성에 미치는 영향 |
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이수윤ㆍ김재우 |
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Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea |
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| Abstract |
PURPOSE
To investigate the effects of dexamethasone (DEX) on the production of nitric oxide (NO) and its enzymatic synthetic pathway in cultured human trabecular meshwork (HTM) cells. METHODS: Primarily cultured HTM cells were exposed to 0, 10, 100, 1000 nM of DEX for 3 days. In addition, 100 micrometer sepiapterin, 100 micrometer ascorbic acid, and 10 micrometer methotrexate were co-exposed to DEX. The cellular survival and nitrite production rates were assessed by MTT assay and Griess assay, respectively. RESULTS: DEX did not significantly affect the survival of cultured HTM cells. DEX decreased the NO production in a dose-dependent manner. With co-exposure of DEX, ascorbic acid nullified the DEX-induced decrease of NO production. Sepiapterin and methotrexate did not affect DEX-induced decrease of NO production. CONCLUSIONS: DEX decreased NO production in HTM cells and the de novo pathway of tetrahydrobiopterin may be involved. This decrease may raise intraocular pressure by decreasing trabecular outflow. |
| Key Words:
Dexamethasone;Nitric oxide;Tetrahydrobiopterin;Trabecular meshwork cells |
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