| Effect of High Glucose on the Oxidative Stress in Trabecular Meshwork Cells. |
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Jae Woo Kim, Chang Beum Bae, Jeong Hun Lee |
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Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea. jwkim@cu.ac.kr |
| 고포도당이 섬유주세포의 산화스트레스에 미치는 영향 |
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김재우ㆍ배창범ㆍ이정훈 |
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Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea |
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| Abstract |
PURPOSE
To investigate the effect of high glucose (HG) on the oxidative stress in cultured human trabecular meshwork cells (HTMC). METHODS: Primarily cultured HTMC were exposed to low glucose (5 mM) and HG (25 mM) for 7 days. Additionally, 1 mM L-arginine, 5 mM DAHP, 10 microgram/ml insulin, 100 micrometer L-ascorbic acid, 10, and 100 micrometer sepiapterin were co-exposed. The cellular survival and nitric oxide (NO) production were assessed by MTT assay and Griess assay, respectively. Superoxide production was measured by modified cytochrome c assay. RESULTS: HG did not affect the survival of cultured HTMC significantly. HG decreased NO production. Co-exposed DAHP decreased but DAHP and insulin increased NO production. In addition, HG increased superoxide production, which was decreased by insulin, L-ascorbic acid, and sepiapterin. CONCLUSIONS: HG decreased NO production accompanied with increased superoxide production in HTMC. Thus HG induces oxidative stress in HTMC and may cause cellular dysfunction and damage of the trabecular meshwork. |
| Key Words:
Glucose;Nitric oxide;Oxidative stress;Superoxide;Trabecular meshwork cells |
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