Journal of the Korean Ophthalmological Society 2007;48(3):423-430.
Published online March 31, 2007.
Effects of Damaged Human Corneal Epithelial Cells on Differentiation of Human Mesenchymal Stem Cell.
Mi Sun Shin, Hyun Sook Hong, Young Sook Son, Jae Chan Kim
1Department of Ophthalmology Chung-ang University College of Medicine, Seoul Korea. jck50ey@kornet.net
2Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Sciences, Seoul, Korea.
손상 받은 각막 상피세포가 중배엽 줄기세포의 분화에 미치는 영향
신미선1,홍현숙2,손영숙2,김재찬1
Department of Ophthalmology Chung-ang University College of Medicine1, Seoul Korea Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Sciences2, Seoul, Korea
Correspondence:  Jae Chan Kim, M.D.1
Abstract
PURPOSE
To identify the effects of microenvironmental changes caused by human corneal epithelial damages to characteristics or differentiation of human mesenchymal stem cells (hMSCs). METHODS: Artificial corneal damage was induced onto a cultured monolayer of human corneal epithelial cells. hMSCs were then co-cultured with damaged human corneal epithelial cells (dIHCE). Morphological changes in the co-cultured hMSCs were observed. To elucidate the differentiation of hMSCs into corneal keratocytes or epithelial cells, the expressions of alpha-smooth muscle actin, keratin-3/-12, and E-cadherin were confirmed by immunofluorescence. RESULTS: hMSCs co-cultured with dIHCE showed enhanced adherence in the neighborhood of dIHCE and morphological change into dendritic shapes at 6 days post-seeding. Although the expression of alpha-smooth muscle actin, known as hMSCs marker, significantly decreased at the dIHCE-contacted site of hMSCs; there were no expressional changes on keratin-3/-12 and E-cadherin, the markers of corneal epithelial cells. Interestingly, positive expression of corneal epithelial marker keratin-3/-12 was observed in dIHCE co-cultured hMSCs. hMSCs co-cultured with normal human corneal epithelial cells (nIHCE) were unable to attach, and showed no change in the expression of alpha-smooth muscle actin. CONCLUSIONS: It is proposed that dIHCE causes a morphological change in hMSCs, and decreased expression of alpha-smooth muscle actin. These results suggest that dIHCE can affect a change in the characteristics and differentiation of hMSCs.
Key Words: Mesenchymal stem cell;Cornea epithelial cell;Coculture;alpha-smooth muscle actin


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