Journal of the Korean Ophthalmological Society 2007;48(4):555-562.
Published online April 30, 2007.
Effects of Intrastromal Air Injection Compared to Hydro-injection on Keratocyte Apoptosis.
Min Joung Lee, Hyun Ju Lee, Won Ryang Wee, Jin Hak Lee, Mee Kum Kim
1Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea. kmk9@snu.ac.kr
2Seoul Artificial Eye Center, Clinical Research Institute, Seoul National University Hospital, Seoul, Korea.
3Department of Ophthalmology, Seoul National University Bundang Hospital, Seoungnam, Korea.
각막 간질내 공기 주입과 평형염액 주입이 간질세포 자멸사에 미치는 영향 비교
이민정1,2,이현주1,2,위원량1,2,이진학1,2,3,김미금1,2
Department of Ophthalmology, Seoul National University College of Medicine1, Seoul, Korea Seoul Artificial Eye Center, Clinical Research Institute, Seoul National University Hospital2, Seoul, Korea Department of Ophthalmology, Seoul National University Bundang Hospital3, Seoungnam, Korea
Correspondence:  Min Joung Lee, M.D.1,2
Abstract
PURPOSE
To evaluate the effects of intrastromal air injection and intrastromal balanced salt solution (BSS) injection on corneal keratocyte apoptosis. METHODS: Twelve right eyes of New Zealand White rabbits were divided into an air-injected group (n=6) and a hydro-injected group (n=6). Contralateral eyes served as a control. Air or Balanced salt solution (BSS(R), Alcon, USA) was injected into the deep corneal stroma at the paracentral area to propagate into nearly the entire cornea. To reduce the intraocular pressure, anterior chamber paracentesis was performed. The animals were sacrificed 4 hours (n=6) and 24 hours (n=6) after surgery. Central cornea buttons were retrieved to stain with Hematoxylin & Eosin and TUNEL (Apoptag(R), Chemicon). The mean number of apoptotic keratocytes was counted in 24.67+/-4.04 consecutive high power field (HPF). RESULTS: The mean number of TUNEL-positive cells at 4 hours was 12.85+/-7.25/HPF and 0.25+/-0.44/HPF in air-injected and hydro-injected eyes, respectively. It was reduced to 6.25+/-4.02/HPF and 0.15+/-0.37/HPF in air-injected and hydro-injected eyes after 24 hours. The air-injected group showed significantly more TUNEL-positive cells compared with the hydro-injected or control group until 24 hours (p=0.001, p=0.001, Mann-Whitney U test). CONCLUSIONS: Intrastromal air injection induces significant apoptosis of keratocytes suggesting some damages in the peripheral cornea when used in deep lamellar keratoplasty.
Key Words: Apoptosis;Cornea;Deep lamellar keratoplasty;Keratocyte


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