J Korean Ophthalmol Soc > Volume 49(2); 2008 > Article
Journal of the Korean Ophthalmological Society 2008;49(2):340-351.
DOI: https://doi.org/10.3341/jkos.2008.49.2.340    Published online February 29, 2008.
The Calretinin Immunoreactive Ganglion Cell Postsynaptic to the ON-Cholinergic Amacrine Cell in the Guinea Pig.
Hyung Chung Kim, Wool Suh, Jung Il Moon, Kyu Ryong Choi
1Hangil Eye Hospital, Incheon, Korea.
2Department of Ophthalmology, College of Medicine, Ewha Womans University, Seoul, Korea. ckrey02@ewha.ac.kr
3Deparment of Ophthalmology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
기니픽 망막에서 개시형 콜린성 아마크린세포에 연접하는 칼레티닌 면역반응성 신경절 세포
김형정1ㆍ서울2ㆍ문정일3ㆍ최규룡2
"Hangil Eye Hospital1, Incheon, Korea Department of Ophthalmology, College of Medicine, Ewha Womans University2, Seoul, Korea Deparment of Ophthalmology, College of Medicine, The Catholic University of Korea3, Seoul, Korea"
Correspondence:  Hyung-Chung Kim, M.D.1
Abstract
PURPOSE
To demonstrate the characterization calretinin-immunoreactive displaced amacrine cells in the ganglion cell layer using immunohistochemistry and electron microscopy. METHODS: For immunohistochemistry, sections from guinea pig retina were incubated with mouse monoclonal antibody directed against calretinin. For double label studies, sections were incuated in mixture of mouse monoclonal anti-calretinin or rabbit polyclonal anti-calretinin with following antibodies: goat polyclonal anti-ChAT, rabbit polyclonal anti-GABA, mouse monoclonal anti-GABAA receptor alpha1, beta2/3. Sections were analyzed using Bio-rad Radiance Plus confocal scanning microscope. Stained sections from three guinea pig were observed with transmission electron microscope. RESULTS: Calretinin immunoreactivity was present in displaced amacrine cells and ganglion cells gaving rise to processes ramified in the inner part of the inner plexiform layer in stratum 4. The same stratum was also occupied by the dendrites of ON-cholinergic amacrine cells. Double-labeling demonstrated that dendrites and cell bodies of displaced amacrine cells colocalized with ON-cholinergic amacrine cells and dendrites of ganglion cells directly overlapped with dendrites of ON-cholinergic amacrine cells. The synaptic connectivity was identified by electron microscopy. Ganglion cell dendrites received synaptic input from ON-cholinergic amacrine cell. GABAA receptor beta2/3 subunit bands cofaciculates the dendrites of displaced amacrine cell and ganglion cell that are juxtapose to the alpha1 subunit of GABAA receptor. CONCLUSIONS: These results indicate that ON-cholinergic amacrine cells modulate calretinin-labeled ganglion cell via GABAA receptor beta2/3 in the guinea pig retina.
Key Words: Amacrine cell;Calretinin;Ganglion cell;Immunohistochemistry
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