| Effect of Collagen Gel with Growth Factor and Wrapping Materials on Fibrovascularization of Porous Orbital Implant. |
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Joon Young Lee, Suk Young Kim, Chang Kook You, Moo Seok Lee, Sang Yeul Lee, Jung Hyub Oh, Sung Joo Kim |
1Department of Ophthalmology, Kim's Eye Hospital, Myung Gok Eye Research Institute, Seoul, Korea.
2School of Materials Science and Engineering, Kyongbuk, Korea.
3Lucid Korea Ltd., Kyongbuk, Korea.
4Department of Ophthalmology, Yonsei University, College of Medicine, Seoul, Korea.
5Department of Ophthalmology, Inha University, College of Medicine, Inchon, Korea.
6Department of Ophthalmology, Konyang University, College of medicine, Daejon, Korea. cybereyedoc@hanmail.net |
| 콜라겐 젤과 성장인자 전처치 및 피복재료가 다공성안와삽입물의 섬유혈관증식에 미치는 효과 |
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이준영1,김석영2,유창국2,이무석3,이상렬4,오중협5,김성주6 |
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Department of Ophthalmology, Kim`s Eye Hospital, Myung Gok Eye Research Institute,1 Seoul, Korea School of Materials Science and Engineering,2 Kyongbuk, Korea, Lucid Korea Ltd.,3 Kyongbuk, Korea Department of Ophthalmology, Yonsei University, College of Medicine,4 Seoul, Korea, Department of Ophthalmology, Inha University, College of Medicine,5 Inchon, Korea Department of Ophthalmology, Konyang University, College of medicine,6 Daejon, Korea |
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Correspondence:
Joon-Young Lee, M.D.,1 |
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| Abstract |
PURPOSE
To determine the effect of bFGF complexed collagen gel, which allows constant release of bFGF along with biodegradation of the collagen gel. The specific study purpose was to determine whether it can accelerate the fibrovascular ingrowth into wrapped HA-coated porous alumina and to verify the safety of new wrapping materials. Synthetic polyester-urethane (Neuropatch(R)) and lyophilized bovine pericardium (Lyoplant(R)) were compared to donor sclera for the fibrovascular ingrowth into HA-coated porous alumina. METHODS: The experimental and control groups, each consisting of 9 rabbits were wrapped with each wrapping materials (3 rabbits per wrapping material). The experimental group underwent pretreatment of bFGF-collagen gel while the control group did not. The fibrovascular ingrowth was compared at 2 and 4 weeks after implantation. Western blot analysis was conducted at 4 weeks using antibodies against CD141 and laminin. The rate of fibrovascular ingrowth was fastest in orbital implant wrapped with Lyoplant(R). RESULTS: Histopathologic examinations at 2 weeks showed no differences in distance and percentage area of fibrovascular ingrowth. Histopathologic examinations at 4 weeks showed that pretreatment of bFGF-collagen gel increased the fibrovascular ingrowth in the experimental group. Western blot analysis on experimental group also showed that the expressions of CD141 and laminin were increased by bFGF-collagen gel, thereby indicating that the fibrovascular proliferations were accelerated by bFGF released from the complex. CONCLUSIONS: bFGF-collagen gel increased the rate and degree of fibrovascular growth into hydroxyapatite-coated porous alumina by releasing bFGF as the collagen gel biodegraded. Both Lyoplant(R) and Neuropatch(R) were evaluated as safe for substitution of the donor sclera. |
| Key Words:
Basic fibroblast growth factor (bFGF);Collagen gel;Hydroxyapatite-coated porous alumina;Wrapping materials |
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