Journal of the Korean Ophthalmological Society 2006;47(8):1340-1348.
Published online August 31, 2006.
The Effects of Anti-allergic Ophthalmic Agents on the Cultured Rabbit Conjunctival Cells.
Ik Soo Byon, Jae Sung Park, Ji Eun Lee, Jong Soo Lee
Department of Ophthalmology, College of Medicine, Pusan National University, Busan, Korea. jongsool@pusan.ac.kr
항알러지 점안약이 배양된 가토의 결막세포에 미치는 영향
변익수,박재성,이지은,이종수
Department of Ophthalmology, College of Medicine, Pusan National University, Busan, Korea
Correspondence:  Ik Soo Byon, M.D.
Abstract
PURPOSE
To investigate the biological effects and cytotoxicity of anti-allergic ophthalmic agents on the cultured conjunctival cells of rabbit in vitro. METHODS: Conjunctival cells of rabbit were exposed to anti-allergic ophthalmic agents. Azelan(R) (Taejoon, Korea), Zaditen(R) (Novartis, USA), Patanol(R) (Alcon, USA) at a concentration 10, 20 and 30% for a period of 30 minutes, 4, 12, and 24 hours respectively. Cell injury assay was performed using lactate dehydrogenase (LDH) leakage assay. We checked the composition, pH, osmolarity of three anti-allergic agents. Light and transmission electron microscopy were performed to compare the cellular damage of rabbit conjunctival cells under various culture treatments. RESULTS: In cultured conjunctival cells of rabbit, the LDH titers increased up to 4 hours after exposure, maintained until 12 hours and then decreased 12 hours after exposed. Azelan(R) and Zaditen(R) showed a higher LDH titer and severe cellular damage of the conjunctival cells, compared with Patanol(R). Of anti-allergic solutions, Azelan(R) and Zaditen(R) revealed markedly lower Na+, Cl- and pH levels than Patanol(R). However, there was no difference in the concentration of preservative or osmolarity of the eye solution among the three anti-allergic agents. CONCLUSIONS: Patanol(R) caused markedly more damage to cultured rabbit conjunctival cells than Azelan(R) and Zaditen(R). If these anti-allergic these agents are clinically used for long periods of time, they may induce the cellular damage of conjunctival cells depending on the composition and pH of anti-allergic drugs.
Key Words: Anti-allergic agents;Azelan(R);Conjunctival cells of rabbit;LDH;Patanol(R);Zaditen(R)


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