| The Characteristics of Limbal Epithelial Cells Auto-Cultivated In Vivo on Amniotic Membrane in Rabbits. |
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Jung Min Park, Jung Min Woo, Hyun Chul Cheon, Woo Jin Jeung, Woo Chan Park |
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Department of Ophthalmology, Dong-A University College of Medicine, Pusan, Korea. wcpark@daunet.donga.ac.kr |
| 가토의 생체내에서 양막위에 배양된 자가 각막윤부상피세포의 특성 |
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박정민,우정민,천현철,정우진,박우찬 |
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Department of Ophthalmology, Dong-A University College of Medicine, Pusan, Korea |
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Correspondence:
Jung Min Park, M.D. |
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| Abstract |
PURPOSE
To investigate the characteristics of the limbal epithelial cells auto-cultivated in vivo on amniotic membrane (LIVAMs) designed for the treatment of limbal stem cell deficiency. METHODS: We removed the epithelium of AM with a No.15 blade after it was blotted with 20% ethanol and made a 360 degrees stromal flap along the epithelial defect. We then mounted over-sized AM (1 mm larger in diameter than the defect) over the defect with the border of AM inserted under the flap, and performed interrupted suture with 10-0 nylon. A therapeutic contact lens was fitted over the AM and a temporary tarsorrhaphy was performed. To examine whether the limbal epithelial cells grew well onto AM, we observed the cornea after fluorescein dye staining using a slit lamp. To explore the characteristics of LIVAMs, we performed hematoxylin-eosin (H&E) staining, immunochemical staining with AK-2, AE-5, AM-3 monoclonal antibodies, and transmission electron microscopy. RESULTS: Three of four rabbits had successful epithelial growth on the amniotic membrane. The epithelial growth on the amniotic membrane was stained using immunohistochemical staining (AK-2, AE-5). Electron microscopy showed a structure similar to that of a normal corneal epithelium. CONCLUSIONS: The technique of auto-cultivation of limbal epithelial cells in vivo on amniotic membrane can be an efficient and convenient method and preserves the characteristics of limbal epithelial cells for the treatment of limbal stem cell deficiency. |
| Key Words:
Amniotic membrane;In vivo culture;Limbal epithelial cell |
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